Šorli Jernej, Lenasi Helena
General Hospital Dr. Franc Derganc, 5290 Šempeter pri Gorici, Slovenia.
Institute of Physiology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000 Ljubljana, Slovenia.
Life (Basel). 2023 Dec 28;14(1):56. doi: 10.3390/life14010056.
We aimed to elucidate the effects of acute hyperglycaemia, induced by an oral glucose tolerance test (OGTT), on the autonomic nervous system (ANS) and skin microvascular reactivity at the time point of peak plasma glucose concentration (c) in 20 young, healthy participants. We assessed their heart rate variability (HRV) as a measure of the ANS activity and the parameters of post-occlusive reactive hyperaemia (PORH) to estimate skin microvascular reactivity as measured by laser Doppler (LD) fluxmetry. The tests were repeated 30 min after a standard OGTT (75 g glucose dissolved in 250 mL water) and, in a separate control experiment, after drinking the same amount of water. Participants had their c and serum insulin measured at three consecutive time-points according to the testing protocol. The low-frequency (LF) spectral power, the LF to high-frequency (LF/HF) ratio, and the diastolic blood pressure increased significantly more after water than after OGTT, and there was a trend of the peak LD flux of PORH decreasing more after OGTT than after water. Significant correlations between some PORH and all the HRV parameters and c increase after OGTT were found, implying diminished vascular reactivity evoked by hyperglycaemia in healthy subjects with lower glucose tolerance.
我们旨在阐明口服葡萄糖耐量试验（OGTT）诱发的急性高血糖对20名年轻健康参与者在血浆葡萄糖浓度峰值（c）时间点的自主神经系统（ANS）和皮肤微血管反应性的影响。我们评估了他们的心率变异性（HRV）作为ANS活动的指标，并通过激光多普勒（LD）血流仪测量的闭塞后反应性充血（PORH）参数来估计皮肤微血管反应性。在标准OGTT（75克葡萄糖溶解于250毫升水中）后30分钟重复进行测试，并在另一个对照实验中，在饮用相同量的水后进行测试。根据测试方案，在三个连续时间点测量参与者的c和血清胰岛素。低频（LF）谱功率、LF与高频（LF/HF）比值以及舒张压在饮水后比OGTT后显著增加更多，并且PORH的LD通量峰值在OGTT后比饮水后下降更多存在趋势。发现OGTT后一些PORH与所有HRV参数以及c升高之间存在显著相关性，这意味着在葡萄糖耐量较低的健康受试者中，高血糖诱发的血管反应性降低。
