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鉴定抗瓜类枯萎病菌 KN2 生理小种的基因。

Identification of Gene Responsible for Conferring Resistance against Race KN2 of in Melon.

机构信息

Department of Horticulture, Sunchon National University, Suncheon 57922, Republic of Korea.

出版信息

Int J Mol Sci. 2024 Jan 17;25(2):1134. doi: 10.3390/ijms25021134.

Abstract

Powdery mildew caused by is a serious fungal disease which causes severe damage to melon production. Unlike with chemical fungicides, managing this disease with resistance varieties is cost effective and ecofriendly. But, the occurrence of new races and a breakdown of the existing resistance genes poses a great threat. Therefore, this study aimed to identify the resistance locus responsible for conferring resistance against race KN2 in melon line IML107. A bi-parental F population was used in this study to uncover the resistance against race KN2. Genetic analysis revealed the resistance to be monogenic and controlled by a single dominant gene in IML107. Initial marker analysis revealed the position of the gene to be located on chromosome 2 where many of the resistance gene against have been previously reported. Availability of the whole genome of melon and its R gene analysis facilitated the identification of a F-box type Leucine Rich Repeats (LRR) to be accountable for the resistance against race KN2 in IML107. The molecular marker developed in this study can be used for marker assisted breeding programs.

摘要

由 引起的白粉病是一种严重的真菌病害,会对瓜类生产造成严重破坏。与化学杀菌剂不同,使用抗性品种来管理这种疾病具有成本效益和环保性。但是,新菌系的出现和现有抗性基因的失效对其构成了巨大威胁。因此,本研究旨在鉴定导致甜瓜品系 IML107 对 菌系 KN2 产生抗性的抗性基因座。本研究使用了双亲 F1 群体来揭示对 KN2 菌系的抗性。遗传分析表明,该抗性是由 IML107 中的单个显性基因控制的单基因。初步标记分析表明,该基因位于 2 号染色体上,此前已有许多针对 菌的抗性基因被报道位于该染色体上。甜瓜全基因组的可用性及其 R 基因分析有助于确定一个 F-box 型亮氨酸重复(LRR)是导致 IML107 对 KN2 菌系产生抗性的原因。本研究开发的分子标记可用于标记辅助育种计划。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/349c/10816175/81af9249f1fa/ijms-25-01134-g001.jpg

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