Miranda-Yuquilema José, Taboada Juan, Once Verónica, Coyago Marco, Briñez Wilfrido
Animal Production and Industrialization Research Unit, Engineering Faculty, Universidad Nacional de Chimborazo, Riobamba 060150, Ecuador.
Faculty of Agricultural Sciences, Universidad de Cuenca, Cuenca 010205, Ecuador.
Microorganisms. 2024 Jan 10;12(1):133. doi: 10.3390/microorganisms12010133.
The aim of the study was to evaluate the impact of probiotics obtained from an agroindustrial waste substrate fermented with lactic acid bacteria and/or yeasts on the health and changes in the microbiota of the digestive tract of guinea pigs. Eighty male guinea pigs, Kuri breed, 30 days old and 250 g live weight, were randomly selected and divided into four groups of 20 animals each: T0, control; T1, and ; T2, and and T3. , and . T1, T2 and T3 contained molasses-vinasse substrate in their base, the dose administered was 1.00 mL/animal orally every 3 days. The indicators evaluated were weight gain, occurrence of diarrhea and mortality, macroscopic lesions in the digestive tract organs and changes in the microbiota of the stomach, caecum, small and large intestine. Treatments T1, T2 and T3 improved weight gain ( < 0.05) and reduced the presence of guinea pigs with diarrhea ( < 0.05) and there was no mortality; animals in the control group presented a greater amount ( < 0.05) of macroscopic lesions in the digestive tract organs; in the T1, T2 and T3 groups there was an improvement in the natural microbiota. It is concluded that the inclusion of a microbial additive in young guinea pigs improves intestinal health and consequently improves weight gain, reduces diarrhea and deaths and normalizes the natural microbiota of the gastrointestinal tract.
本研究的目的是评估从用乳酸菌和/或酵母发酵的农业工业废弃底物中获得的益生菌对豚鼠健康及消化道微生物群变化的影响。随机选择80只雄性库里品种豚鼠，30日龄，活重250克，分为四组，每组20只动物：T0为对照组；T1，以及；T2，以及；T3。，以及。T1、T2和T3组以糖蜜酒糟底物为基础，给药剂量为每3天每只动物口服1.00毫升。评估的指标包括体重增加、腹泻和死亡率、消化道器官的宏观病变以及胃、盲肠、小肠和大肠微生物群的变化。T1、T2和T3组改善了体重增加（<0.05），减少了腹泻豚鼠的数量（<0.05），且无死亡情况；对照组动物消化道器官的宏观病变数量更多（<0.05）；T1、T2和T3组的自然微生物群有所改善。得出的结论是，在幼年豚鼠中添加微生物添加剂可改善肠道健康，从而促进体重增加，减少腹泻和死亡，并使胃肠道的自然微生物群正常化。
