Azad Md Thoufic Anam, Sugi Tatsuki, Qulsum Umme, Kato Kentaro
Laboratory of Sustainable Animal Environment, Graduate School of Agricultural Science, Tohoku University, 232-3 Yomogida, Naruko-onsen, Osaki, Miyagi 989-6711, Japan.
Department of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi 6205, Bangladesh.
Microorganisms. 2024 Jan 10;12(1):137. doi: 10.3390/microorganisms12010137.
Transcriptional variation has been studied but post-transcriptional modification due to RNA editing has not been investigated in . We investigated developmental stage-specific RNA editing in selected genes in 3D7. We detected extensive amination- and deamination-type RNA editing at 8, 16, 24, 32, 40, and 46 h in tightly synchronized . Most of the editing events were observed in 8 and 16 h ring-stage parasites. Extensive A-to-G deamination-type editing was detected more during the 16 h ring stage (25%) than the 8 h ring stage (20%). Extensive U-to-C amination-type editing was detected more during the 16 h ring stage (31%) than the 8 h ring stage (22%). In 28S, rRNA editing converted the loop structure to the stem structure. The hemoglobin binding activity of PF3D7_0216900 was also altered due to RNA editing. Among the expressed 28S rRNA genes, PF3D7_0532000 and PF3D7_0726000 expression was higher. Increased amounts of the transcripts of these two genes were found, particularly PF3D7_0726000 in the ring stage and PF3D7_0532000 in the trophozoite and schizont stages. Adenosine deaminase (ADA) expression did not correlate with the editing level. This first experimental report of RNA editing will help to identify the editing machinery that might be useful for antimalarial drug discovery and malaria control.
转录变异已被研究，但RNA编辑导致的转录后修饰在[具体对象未提及]中尚未被研究。我们研究了3D7中选定基因的发育阶段特异性RNA编辑。我们在紧密同步的[具体对象未提及]中于8、16、24、32、40和46小时检测到广泛的胺化和脱氨型RNA编辑。大多数编辑事件在8和16小时的环状阶段寄生虫中观察到。在16小时环状阶段检测到的广泛的A到G脱氨型编辑（25%）比8小时环状阶段（20%）更多。在16小时环状阶段检测到的广泛的U到C胺化型编辑（31%）比8小时环状阶段（22%）更多。在28S中，rRNA编辑将环结构转变为茎结构。PF3D7_0216900的血红蛋白结合活性也因RNA编辑而改变。在表达的28S rRNA基因中，PF3D7_0532000和PF3D7_0726000的表达较高。发现这两个基因的转录本数量增加，特别是环状阶段的PF3D7_0726000和滋养体及裂殖体阶段的PF3D7_0532000。腺苷脱氨酶（ADA）的表达与编辑水平无关。这份关于RNA编辑的首次实验报告将有助于识别可能对抗疟药物发现和疟疾控制有用的编辑机制。
