Mahamat Djamalladine Djamalladine, Mabou Florence Déclaire, Feugap Tsamo Donald Leonel, Tamokou Jean-De-Dieu, Voutquenne-Nazabadioko Laurence, Tsopmo Apollinaire, Ngnokam David
Research Unit of Environmental and Applied Chemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
Research Unit of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, Dschang, Cameroon.
Nat Prod Res. 2025 Jan;39(2):362-373. doi: 10.1080/14786419.2023.2301682. Epub 2024 Jan 23.
The chemical investigation of the aerial part of led to isolation of a new triterpenoid glycoside named Canescensoside () and four known compounds including longispinogenin-3---D-glucuronopyranoside (), -sitosterol-3---D-glucoside (), apigenin-7---D-glucopyranoside () and apigenin-7--[-L-rhamnopyranosyl-(1→3)--D-glucopyranoside] (). Structures of compounds were assigned by interpretation of their spectral data, mainly 1D and 2D NMR, HRESIMS, and by comparison with the reported data. The MeOH extract, EtOAc and -BuOH fractions as well as isolated compounds were tested for their antibacterial activities against four bacteria strains among which, two Gram-negative ( ATCC 76110 and ATCC 8739) and two Gram-positive ( ATCC 29212 and ATCC 25923) bacteria using the broth microdilution method. The MeOH extract and EtOAc fraction exhibited significant activities (MIC values ranging from 128 to 512 g/mL) against all the tested bacteria. Compounds and showed the lowest MIC values of 55.47 and 50.40 M, respectively.
对[植物名称]地上部分进行化学研究，从中分离出一种名为Canescensoside（）的新三萜糖苷以及四种已知化合物，包括长刺皂苷元-3---D-葡萄糖醛酸吡喃糖苷（）、β-谷甾醇-3---D-葡萄糖苷（）、芹菜素-7---D-葡萄糖吡喃糖苷（）和芹菜素-7--[-L-鼠李糖吡喃糖基-(1→3)--D-葡萄糖吡喃糖苷]（）。通过对其光谱数据（主要是一维和二维核磁共振、高分辨电喷雾电离质谱）的解析，并与已报道数据进行比较，确定了化合物的结构。采用肉汤微量稀释法，对甲醇提取物、乙酸乙酯和正丁醇馏分以及分离得到的化合物针对四种细菌菌株进行了抗菌活性测试，其中包括两种革兰氏阴性菌（[细菌名称1] ATCC 76110和[细菌名称2] ATCC 8739）以及两种革兰氏阳性菌（[细菌名称3] ATCC 29212和[细菌名称4] ATCC 25923）。甲醇提取物和乙酸乙酯馏分对所有测试细菌均表现出显著活性（最低抑菌浓度值范围为128至512μg/mL）。化合物[化合物名称1]和[化合物名称2]的最低抑菌浓度值分别为55.47和50.40μM，为最低。
