Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Hezar-Jereeb Street, Isfahan, 81746-73441, Iran.
Sci Rep. 2024 Jan 23;14(1):2007. doi: 10.1038/s41598-023-50916-5.
The most frequent infections caused by Pseudomonas aeruginosa are local infections in soft tissues, including burns. Today, phage use is considered a suitable alternative to cure infections caused by multi-drug-resistant (MDR) and extensively drug-resistant (XDR) bacteria. We investigated the potential of a novel phage (vB_PaS-HSN4) belonging to Caudoviricetes class, against XDR and MDR P. aeruginosa strains in vivo and in vitro. Its biological and genetic characteristics were investigated. The phage burst size and latent were 119 and 20 min, respectively. It could tolerate a broad range of salt concentrations, pH values, and temperatures. The combination with ciprofloxacin significantly enhanced biofilm removal after 24 h. The genome was dsDNA with a size of 44,534 bp and encoded 61 ORFs with 3 tRNA and 5 promoters. No virulence factor was observed in the phage genome. In the in vivo infection model, treatment with vB_PaS-HSN4 increased Galleria mellonella larvae survival (80%, 66%, and 60%) (MOI 100) and (60%, 40%, and 26%) (MOI 1) in the pre-treatment, co-treatment, and post-treatment experiments, respectively. Based on these characteristics, it can be considered for the cure of infections of burns caused by P. aeruginosa.
铜绿假单胞菌引起的最常见感染是软组织局部感染，包括烧伤。如今，噬菌体的使用被认为是治疗多药耐药（MDR）和广泛耐药（XDR）细菌感染的合适替代品。我们研究了一种新型噬菌体（vB_PaS-HSN4）在体内和体外对抗 XDR 和 MDR 铜绿假单胞菌菌株的潜力。研究了其生物学和遗传特性。噬菌体的爆发量和潜伏期分别为 119 和 20 分钟。它可以耐受广泛的盐浓度、pH 值和温度。与环丙沙星联合使用可显著提高生物膜在 24 小时后的清除率。基因组为双链 DNA，大小为 44534bp，编码 61 个 ORF，其中包括 3 个 tRNA 和 5 个启动子。噬菌体基因组中未观察到毒力因子。在体内感染模型中，vB_PaS-HSN4 处理分别提高了大蜡螟幼虫的存活率（80%、66%和 60%）（MOI 100）和（60%、40%和 26%）（MOI 1）的预治疗、共同治疗和后治疗实验。基于这些特性，它可以考虑用于治疗铜绿假单胞菌引起的烧伤感染。
