Vincent Scott M, Madani Melika, Dikeman Dante, Golden Kyle, Crocker Naomi, Jackson Cameron, Wimmer Sam P, Dover Mary, Tucker Alexis, Ghiani Cristina A, Colwell Christopher S, LeBaron Tyler W, Tarnava Alex, Paul Ketema N
Department of Integrative Biology and Physiology, University of California Los Angeles, Los Angeles, CA, USA.
Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
Sleep Adv. 2023 Dec 30;5(1):zpad057. doi: 10.1093/sleepadvances/zpad057. eCollection 2024.
Sleep loss contributes to various health issues and impairs neurological function. Molecular hydrogen has recently gained popularity as a nontoxic ergogenic and health promoter. The effect of molecular hydrogen on sleep and sleep-related neural systems remains unexplored. This study investigates the impact of hydrogen-rich water (HRW) on sleep behavior and neuronal activation in sleep-deprived mice.
Adult C57BL/6J mice were implanted with electroencephalography (EEG) and electromyography (EMG) recording electrodes and given HRW (0.7-1.4 mM) or regular water for 7 days ad libitum. Sleep-wake cycles were recorded under baseline conditions and after acute sleep loss. Neuronal activation in sleep- and wake-related regions was assessed using cFos immunostaining.
HRW increased sleep consolidation in undisturbed mice and increased non-rapid-eye movement and rapid-eye-movement sleep amount in sleep-deprived mice. HRW also decreased the average amount of time for mice to fall asleep after light onset. Neuronal activation in the lateral septum, medial septum, ventrolateral preoptic area, and median preoptic area was significantly altered in all mice treated with HRW.
HRW improves sleep consolidation and increases neuronal activation in sleep-related brain regions. It may serve as a simple, effective treatment to improve recovery after sleep loss.
睡眠不足会导致各种健康问题并损害神经功能。分子氢作为一种无毒的促力剂和健康促进剂，近来颇受关注。分子氢对睡眠及与睡眠相关的神经系统的影响尚不清楚。本研究调查富氢水（HRW）对睡眠剥夺小鼠睡眠行为和神经元激活的影响。
成年C57BL/6J小鼠植入脑电图（EEG）和肌电图（EMG）记录电极，并随意给予HRW（0.7 - 1.4 mM）或普通水7天。在基线条件下和急性睡眠剥夺后记录睡眠-觉醒周期。使用cFos免疫染色评估睡眠和觉醒相关区域的神经元激活情况。
HRW增加了未受干扰小鼠的睡眠巩固，并增加了睡眠剥夺小鼠的非快速眼动睡眠和快速眼动睡眠量。HRW还减少了小鼠在光照开始后入睡的平均时间。在所有接受HRW治疗的小鼠中，外侧隔区、内侧隔区、腹外侧视前区和视前正中区的神经元激活均有显著改变。
HRW改善睡眠巩固，并增加与睡眠相关脑区的神经元激活。它可能是一种简单有效的治疗方法，可改善睡眠剥夺后的恢复情况。
