Li Xiaolan, Hu Huan, Ren Qunli, Wang Miao, Du Yimei, He Yuqi, Wang Qian
Microbial Resources and Drug Development Key Laboratory of Guizhou Tertiary Institution, Life Sciences Institute, School of Stomatology, Zunyi Medical University, Zunyi 563000, China.
School of Pharmacy, Zunyi Medical University, Zunyi 563000, China.
Plant Biotechnol (Tokyo). 2023 Jun 25;40(2):145-155. doi: 10.5511/plantbiotechnology.23.0208a.
usually lives on rock or tree, but their endophyte diversity has not yet been fully revealed? In this study, high-throughput sequencing technology was used to investigate the endophyte diversity of the roots of lived on tree (Group 1-3, arboreal type) and rock (Group 4, lithophytic type). The results showed that their composition of endophytic fungi and bacteria were similar at phylum level, while their relative abundance were different. Their taxa composition and abundance of endophytes differed significantly among groups at the genus level. diversity of endophytic fungi of lithophytic type was higher than those from arboreal type, while there was no advantage in endophytic bacteria. diversity revealed that the endophytic fungi tended to cluster in each group, but the endophytic bacteria were dispersed among the groups. LEfSe analysis found that the numbers of predicted endophyte biomarkers of lithophytic type were more than arboreal types at genus level, and the biomarkers varied among groups. Microbial network analysis revealed similarities and differences in the taxa composition and abundance of shared and special endophytes in each group. These results suggested that the root endophytes of lithophytic and arboreal differed in diversity.
通常生活在岩石或树上，但其内生菌多样性尚未完全揭示？在本研究中，采用高通量测序技术研究了生活在树上（第1 - 3组，树栖型）和岩石上（第4组，石生型）的植物根系内生菌多样性。结果表明，它们的内生真菌和细菌在门水平上组成相似，但相对丰度不同。在属水平上，它们内生菌的分类群组成和丰度在组间存在显著差异。石生型内生真菌的多样性高于树栖型，而内生细菌方面则没有优势。多样性分析表明，内生真菌倾向于在每组中聚类，但内生细菌在各组间分散分布。LEfSe分析发现，在属水平上，石生型预测的内生菌生物标志物数量多于树栖型，且生物标志物在组间存在差异。微生物网络分析揭示了每组中共享和特殊内生菌在分类群组成和丰度上的异同。这些结果表明，石生型和树栖型植物的根内生菌在多样性上存在差异。
