Department of Pharmacology and Neuroscience, Creighton University School of Medicine, Omaha, NE 68178, USA.
Sanofi, Rare and Neurologic Diseases, Cambridge, MA, USA.
STAR Protoc. 2023 Dec 15;4(4):102630. doi: 10.1016/j.xpro.2023.102630. Epub 2023 Oct 9.
Global cerebral ischemia occurs when blood flow to the entire brain is transiently blocked, which results in delayed neurologic deficits. Here, we present a protocol for performing the four-vessel occlusion rat model to study the neurodegeneration and cognitive deficits associated with global ischemia. We describe steps for carrying out the vertebral and common carotid artery occlusion which enables sufficient blockage of cerebral blood flow. We then detail expected outcomes using histology assays and behavioral tests. For complete details on the use and execution of this protocol, please refer to Chung et al. (2022)..
当血流暂时阻断整个大脑时，会发生全脑缺血，导致神经功能缺损延迟。在这里，我们提供了一个使用四血管闭塞法建立大鼠全脑缺血模型的方案，用于研究与全脑缺血相关的神经退行性变和认知功能障碍。我们描述了进行椎动脉和颈总动脉闭塞的步骤，这些步骤可充分阻断脑血流。然后，我们详细介绍了使用组织学检测和行为测试的预期结果。如需了解本方案的详细使用和实施，请参考 Chung 等人（2022 年）。
