Kaufman B M, Summers P L, Dubois D R, Eckels K H
Am J Trop Med Hyg. 1987 Mar;36(2):427-34. doi: 10.4269/ajtmh.1987.36.427.
A panel of 11 murine monoclonal antibodies directed against dengue type 2 was evaluated for antigen specificity by dot immunobinding assay and Western blot analysis and for in vitro and in vivo biological activities. Nine of the 11 monoclonal antibodies reacted with viral E-glycoprotein based on the Western blot analysis; one reacted with a 36 Kd protein present in dengue-infected C6/36 mosquito cells. The nine E-glycoprotein-reactive monoclonal antibodies also neutralized dengue 2 virus in a plaque reduction assay. Of the neutralizing monoclonal antibodies, five passively protected mice in vivo against lethal intracerebral dengue 2 challenge. The protective monoclonal antibodies were directed against viral determinants that fell into at least three spatially separate families of epitopes on E-glycoprotein, the antigenicities of which were preserved after heat/detergent denaturation.
通过斑点免疫结合试验和蛋白质印迹分析评估了一组针对2型登革热的11种鼠单克隆抗体的抗原特异性，并评估了其体外和体内生物学活性。根据蛋白质印迹分析，11种单克隆抗体中有9种与病毒E糖蛋白反应；1种与登革热感染的C6/36蚊细胞中存在的一种36 Kd蛋白反应。这9种与E糖蛋白反应的单克隆抗体在蚀斑减少试验中也中和了2型登革热病毒。在中和性单克隆抗体中，有5种在体内被动保护小鼠免受致死性脑内2型登革热攻击。保护性单克隆抗体针对的是病毒决定簇，这些决定簇至少属于E糖蛋白上三个空间上分开的表位家族，其抗原性在热/去污剂变性后得以保留。
