Herrmann U, Krawielitzki K, Smulikowska S, Schadereit R
Arch Tierernahr. 1986 Oct;36(10):857-65. doi: 10.1080/17450398609425319.
A ration consisting of wheat gluten and N-free components was supplemented with L-lysine and L-leucine and fed to two groups of growing Wistar rats. Group 1 received 15N Lys and unlabelled Leu, group 2 received unlabelled Lys and 15N Leu in order to study the influence of the utilization of the 15N marker on the labelling quota of faeces and urine as well as various fractions of the body. The good utilization of Lys in group 1 results in a higher 15N excess in faeces and a reduced 15N abundance in urine in comparison to group 2 with a lower utilization of 15N Leu. The results show that the 15N abundance in urine is unsuitable as an indicator of the 15N labelling quota of endogenous metabolic faecal nitrogen.
由小麦面筋和无氮成分组成的日粮补充了L-赖氨酸和L-亮氨酸,并喂给两组生长中的Wistar大鼠。第1组接受15N标记的赖氨酸和未标记的亮氨酸,第2组接受未标记的赖氨酸和15N标记的亮氨酸,以研究15N标记物的利用对粪便和尿液以及身体各部分标记配额的影响。与第2组较低的15N亮氨酸利用率相比,第1组赖氨酸的良好利用导致粪便中15N过量较高,尿液中15N丰度降低。结果表明,尿液中的15N丰度不适合作为内源性代谢粪便氮15N标记配额的指标。