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× 扎贝尔‘霍帕’(蔷薇科)的完整叶绿体基因组序列

The complete chloroplast genome sequence of × Zabel 'Hopa' (Rosaceae).

作者信息

Dong Leiming, Wang Ruizhen, Liu Hengxing, Xia Guowei, Quan Jian, Guo Ling, Chen Minghui

机构信息

Key Laboratory of National Forestry and Grassland Administration on Plant Ex situ Conservation, Beijing Floriculture Engineering Technology Research Centre, Beijing Botanical Garden, Beijing, China.

College of Eco-Environmental Engineering, Guizhou Minzu University, Guiyang, China.

出版信息

Mitochondrial DNA B Resour. 2024 Jan 25;9(1):173-177. doi: 10.1080/23802359.2023.2292158. eCollection 2024.

Abstract

× Zabel 'Hopa' is an important crabapple cultivar with significant ornamental value. Here, we assembled its complete chloroplast (cp) genome using the next-generation sequencing technology to clarify the phylogenetic relationships in . The total length of the complete chloroplast genome was 160,230 base pairs (bp) with a GC content of 36.50%, consisting of a large single-copy (LSC) region with a sequence length of 88,310 bp, a small single-copy (SSC) region with a sequence length of 19,196 bp, and a pair of inverted repeat (IR) regions of 26,362 bp. The complete chloroplast genome contained 128 genes, namely 84 protein-coding genes, 36 tRNA genes, and 8 rRNA genes. In addition, 73 SSRs were found in the . 'Hopa' cp genome. The phylogenetic relationship of 'Hopa' in is closely related to (Aiton) Borkh. and then to (Lebed.) M. Roem. Our results demonstrate that it is feasible to resolve the phylogenetic relationships of crabapple cultivars and identify their putative maternal lineages using cp genomic data.

摘要

ד霍帕”海棠是一种具有重要观赏价值的海棠品种。在此,我们利用下一代测序技术组装了其完整的叶绿体(cp)基因组,以阐明其系统发育关系。完整叶绿体基因组的总长度为160,230个碱基对(bp),GC含量为36.50%,由一个长度为88,310 bp的大单拷贝(LSC)区域、一个长度为19,196 bp的小单拷贝(SSC)区域和一对长度为26,362 bp的反向重复(IR)区域组成。完整的叶绿体基因组包含128个基因,即84个蛋白质编码基因、36个tRNA基因和8个rRNA基因。此外,在“霍帕”cp基因组中发现了73个简单序列重复(SSR)。“霍帕”在中的系统发育关系与(艾顿)博尔克密切相关,然后与(列别德)M. 罗伊姆相关。我们的结果表明,利用cp基因组数据解析海棠品种的系统发育关系并确定其推定的母系谱系是可行的。

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