Li Yanan, Liu Yanlei, Xu Chao, Li Fengyi, Wang Ling, Zhou Shiliang
College of Landscape Architecture, Northeast Forestry University, Harbin, China.
State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
Mitochondrial DNA B Resour. 2020 Jul 15;5(3):2826-2827. doi: 10.1080/23802359.2020.1790317.
(Siebold) Siebold ex de Vriese is a worthy species in the genus Malus (family Rosaceae) distributing from North America and Asia, especially in China. In addition to its ornamental value, the medicinal value of its fruits has also been developed to treat food stagnation diseases. Herein, we obtained the complete chloroplast genome of using next-generation sequencing technology. The complete chloroplast genome is 160,039 base pair (bp) in length, including a large single copy (LSC) region of 88,142 bp, a small single copy (SSC) region of 19,183 bp, and a pair of inverted repeat regions (IRs, 26,357 bp). And, the overall GC content of the chloroplast genome was 36.6%. Besides, a total of 128 unique genes were found in the chloroplast genome, namely 83 protein-coding genes (PCGs), 37 tRNA genes (tRNA), and eight rRNA genes (rRNA). A maximum-likelihood phylogenetic tree was reconstructed using the full length of chloroplast genome to show the phylogenetic relationships among species in genus Malus. It was concluded that was phylogenetically close to
(西博尔德海棠)西博尔德海棠(Siebold ex de Vriese)是苹果属(蔷薇科)中一个有价值的物种,分布于北美和亚洲,尤其是中国。除了具有观赏价值外,其果实的药用价值也已被开发用于治疗食积病症。在此,我们利用下一代测序技术获得了[物种名称未给出]的完整叶绿体基因组。完整的叶绿体基因组长度为160,039碱基对(bp),包括一个88,142 bp的大单拷贝(LSC)区域、一个19,183 bp的小单拷贝(SSC)区域和一对反向重复区域(IRs,26,357 bp)。并且,叶绿体基因组的总体GC含量为36.6%。此外,在叶绿体基因组中总共发现了128个独特基因,即83个蛋白质编码基因(PCGs)、37个tRNA基因(tRNA)和8个rRNA基因(rRNA)。利用叶绿体基因组全长重建了最大似然系统发育树,以显示苹果属物种之间的系统发育关系。得出的结论是[物种名称未给出]在系统发育上与[物种名称未给出]关系密切 。