Saari L L, Klucas R V
Biochim Biophys Acta. 1987 Apr 8;912(2):198-202. doi: 10.1016/0167-4838(87)90089-6.
Ferric leghemoglobin isolated from soybean root nodules was reduced nonenzymatically to ferrous leghemoglobin in vitro at pH 5.2 using either 1.0 mM NADH or NADPH as the reductant. In the pH range of 5.2 to 7.0, the highest rates of reduction occurred below pH 6.5 with a maximum rate observed at pH 5.2. Rates of nonenzymatic ferric leghemoglobin reduction above pH 6.5 or at reduced-pyridine nucleotide concentrations below 0.4 mM were insignificant. Oxygen was required for the nonenzymatic reduction. Inhibition of ferric leghemoglobin reduction by superoxide dismutase and catalase indicated that superoxide and hydrogen peroxide may be intermediates in the reaction.
从大豆根瘤中分离出的高铁豆血红蛋白，在体外pH 5.2条件下，以1.0 mM的NADH或NADPH作为还原剂，可被非酶促还原为亚铁豆血红蛋白。在pH 5.2至7.0范围内，还原速率最高值出现在pH 6.5以下，在pH 5.2时观察到最大还原速率。在pH 6.5以上或吡啶核苷酸浓度低于0.4 mM时，非酶促高铁豆血红蛋白的还原速率微不足道。非酶促还原需要氧气。超氧化物歧化酶和过氧化氢酶对高铁豆血红蛋白还原的抑制作用表明，超氧化物和过氧化氢可能是该反应的中间体。
