Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany.
DIL German Institute of Food Technologies, Quakenbrueck, Germany.
Lab Chip. 2024 Feb 13;24(4):975-984. doi: 10.1039/d3lc01103h.
We present a novel centrifugal microfluidic approach to rapidly identify animal species in meat products. The workflow requires a centrifugal cartridge for DNA extraction and for preparation of a recombinant polymerase amplification (RPA) reaction, a programmable centrifuge for processing the cartridge and an isothermal reader to perform the RPA. Liquid reagents are pre-stored on the cartridge and the meat sample can be added directly without any pre-treatment. With this system, we are able to identify six different animal species in a single run within one hour. In pork salami containing horse, turkey, sheep, chicken and beef meat, it was possible to identify species levels as low as 0.01%. In beef salami and cooked pork sausages 0.1% of foreign meat could be detected. This novel workflow enables rapid and sensitive species identification in processed meat at the point of need.
我们提出了一种新颖的离心微流控方法，可快速鉴定肉类产品中的动物物种。该工作流程需要一个用于 DNA 提取和重组聚合酶扩增（RPA）反应制备的离心盒，一个用于处理盒的可编程离心机和一个进行 RPA 的等温读数仪。液体试剂预先储存在盒上，并且可以直接添加肉样而无需任何预处理。使用该系统，我们能够在一个小时内单次运行中鉴定六种不同的动物物种。在含有马、火鸡、羊、鸡和牛肉的猪肉萨拉米中，能够鉴定出低至 0.01%的物种水平。在牛肉萨拉米和熟猪肉香肠中，可以检测到 0.1%的外来肉。这种新颖的工作流程能够在需要的地点快速、灵敏地鉴定加工肉类中的物种。
