f.a.，sp. nov.，一种从花中分离得到的新型酵母。

f.a., sp. nov., a novel yeast isolated from flowers.

机构信息

Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.

Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-49-10 Nishihara, Shibuya, Tokyo 151-0066, Japan.

出版信息

Int J Syst Evol Microbiol. 2024 Jan;74(1). doi: 10.1099/ijsem.0.006253.

DOI:10.1099/ijsem.0.006253

PMID:38284408

Abstract

Three yeast isolates, NBRC 115909, NBRC 115910 and NBRC 116270, were isolated from (red clover) flowers collected from Kisarazu, Chiba, Japan. Analysis of the sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions revealed that these isolates represent a single novel species within the genus . Also, no ascospore formation was observed. The yeast isolates were closely related to UWOPS 00-107.2 and NRRL Y-17081. They differed from , the most closely related species with a validly published name, by ten nucleotide substitutions with two gaps in the D1/D2 domains and 20 nucleotide substitutions in the ITS region. Moreover, the three isolates exhibited distinct phenotypic characteristics from the closely related species. Therefore, we suggest that these three isolates represent a novel species, designated as f.a., sp. nov. The holotype is NBRC 115909 (isotype: CBS 18485).

摘要

三个酵母分离株，NBRC 115909、NBRC 115910 和 NBRC 116270，是从日本千叶市的菊佐拉采集的（红三叶草）花中分离出来的。对大亚基（LSU）rRNA 基因的 D1/D2 结构域和内部转录间隔区（ITS）区域的序列分析表明，这些分离株代表了属内的一个单一新种。此外，也没有观察到子囊孢子的形成。这些酵母分离株与 UWOPS 00-107.2 和 NRRL Y-17081 密切相关。与亲缘关系最近的具有有效发表名称的物种相比，它们在 D1/D2 结构域中有十个核苷酸替换和两个空位，在 ITS 区域中有 20 个核苷酸替换。此外，这三个分离株表现出与亲缘关系密切的物种明显不同的表型特征。因此，我们建议这三个分离株代表一个新的物种，命名为 f.a.，sp. nov. 。模式株是 NBRC 115909（同型：CBS 18485）。