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sp. nov.,一个发现于泰国的子囊菌科中的酵母物种。

sp. nov., an ascomycetous yeast species in the family discovered in Thailand.

机构信息

Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, Thailand.

出版信息

Int J Syst Evol Microbiol. 2024 Apr;74(4). doi: 10.1099/ijsem.0.006330.

Abstract

Four yeast strains, representing a novel anamorphic species, were isolated in Thailand. The two strains (ST-3660 and ST-3647) were obtained from two different estuarine water samples in a mangrove forest. Strain DMKU-FW1-37 was derived from a grease sample, and another strain (TSU57) was isolated from a fruiting body of sp. Pairwise sequence analysis showed that the four strains had identical or differed by only one nucleotide substitution in the D1/D2 domains of the large subunit (LSU) rRNA gene, and differed by one to three nucleotide substitutions in the internal transcribed spacer (ITS) regions. is the most closely related species to the four strains, but with 9-10 (1.57-1.72 %) nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 29-31 (4.22-4.45 %) nucleotide substitutions in the ITS regions. Phylogenetic analyses based on the concatenated sequences of the ITS regions and the D1/D2 domains of the LSU rRNA gene showed that the four strains form a well-separated lineage from with high bootstrap support, confirming that they represent a distinct species. Therefore, the four strains are assigned as representives of a novel species of the genus , for which the name sp. nov. is proposed. The holotype is TBRC 4481 and the ex-type is PYCC 9794 (=ST-3660). The MycoBank number of the novel species is MB 851951.

摘要

四种酵母菌株,代表了一个新的无性型物种,在泰国被分离出来。这两个菌株(ST-3660 和 ST-3647)是从红树林中的两个不同的河口水样中获得的。菌株 DMKU-FW1-37 来自油脂样本,另一个菌株(TSU57)是从 sp 的一个果实体中分离出来的。成对序列分析表明,这四个菌株在大亚基(LSU)rRNA 基因的 D1/D2 结构域中具有相同或仅相差一个核苷酸取代,而在内部转录间隔区(ITS)区域中则相差一个到三个核苷酸取代。 与这四个菌株最密切相关,但在 LSU rRNA 基因的 D1/D2 结构域中存在 9-10(1.57-1.72%)个核苷酸取代,在 ITS 区域中存在 29-31(4.22-4.45%)个核苷酸取代。基于 ITS 区域和 LSU rRNA 基因的 D1/D2 结构域的串联序列的系统发育分析表明,这四个菌株与 形成一个分离良好的谱系,具有高的自举支持,证实它们代表一个独特的物种。因此,这四个菌株被分配为属的一个新种的代表,该新种被命名为 sp. nov.。模式标本是 TBRC 4481,正模式是 PYCC 9794(=ST-3660)。新物种的 MycoBank 编号为 MB 851951。

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