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f.a.,sp. nov.,一种从泰国野生蘑菇中分离出来的新型子囊菌酵母。

f.a., sp. nov., a novel ascomycetous yeast species isolated from wild mushrooms in Thailand.

机构信息

Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.

Biodiversity Center Kasetsart University (BDCKU), Bangkok, 10900, Thailand.

出版信息

Int J Syst Evol Microbiol. 2023 May;73(5). doi: 10.1099/ijsem.0.005840.

DOI:10.1099/ijsem.0.005840
PMID:37167093
Abstract

Two strains of genus , representing a novel anamorphic yeast species, were isolated from two samples of sp. collected in Thailand. Analysis of the sequences of the internal transcribed spacer (ITS) regions and the D1/D2 domains of the large subunit (LSU) rRNA gene showed that the two strains differed by 27-42 nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 7-34 nucleotide substitutions in the ITS region of a group of related species, CBS 11430, JCM 10142, ATCC MYA-4329, SD5S01 and NYNU 167162. Phylogenetic analysis based on the concatenated sequences of the ITS and the D1/D2 domains of the LSU rRNA gene showed that the two strains were placed in the clade and clearly separated from other recognized species of the genus. Therefore, the two strains were assigned as a novel species of the genus , for which we propose the name f.a., sp. nov. The holotype is TBRC 16043 (=DMKU-BP19) and the isotype is PYCC 9354. The MycoBank number of the novel species is MB 846490.

摘要

两株代表一个新型无性酵母种的属菌株,是从泰国采集的两个 sp. 样本中分离出来的。对内部转录间隔区(ITS)和大亚基(LSU)rRNA 基因的 D1/D2 区域的序列分析表明,两株菌株在 LSU rRNA 基因的 D1/D2 区域存在 27-42 个核苷酸取代,在与 CBS 11430、JCM 10142、ATCC MYA-4329、SD5S01 和 NYNU 167162 等相关种的 ITS 区存在 7-34 个核苷酸取代。基于 ITS 和 LSU rRNA 基因的 D1/D2 区域的串联序列的系统发育分析表明,两株菌株被放置在 分支中,并与该属的其他公认种明显分离。因此,两株菌株被分配为该属的一个新型种,我们提议将其命名为 f.a.,sp. nov.。模式株为 TBRC 16043(=DMKU-BP19),等模式株为 PYCC 9354。该新型种的 MycoBank 编号为 MB 846490。

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