Ma Yongyuan, Guo Haiyun, Bai Fuhai, Zhang Ming, Yang Liu, Deng Jiao, Xiong Lize
Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, the Fourth Military Medical University. 127th West Changle Road, Xi'an, 710032, Shaanxi, P.R. China.
General Hospital of Chengdu Military Region of Chinese PLA, the 270th Tianhui Road, Chengdu, 610083, Sichuan, P.R. China.
Exp Anim. 2018 May 10;67(2):271-280. doi: 10.1538/expanim.17-0142. Epub 2018 Jan 1.
Acupuncture is widely used for knee osteoarthritis (KOA) treatment in clinical practice. In the present study, we aimed to set a standard KOA animal model for electroacupuncture (EA) study and provide an acupuncture recipe for further KOA studies. Rats intra-articularly administered monosodium iodoacetate (MIA, 0.3, 1 or 3 mg respectively, n=12 each) were evaluated for pain-like behavior: paw withdrawal mechanical threshold, weight bearing deficit, and joint pathological changes (OARSI score) until 28 days after injury. Then by using the suitable dose (1 mg MIA), therapeutic effects of EA treatment (bilateral ST36 and ST35 acupoints, 2/10 Hz, 30 min/d, 6d/w, 2w) were evaluated in 3 groups (n=16 each): Early-on EA, Mid-term EA and Delayed EA, in which EA was started on day 1, day 7 or day 14 after MIA injection. Both 1 mg and 3 mg MIA induced significant joint damage and persistent pain behavior. But animals accepted 3 mg MIA rapidly developed cartilage and bone damage within 14 days. Early-on EA treatment provided significant pain relief and joint structure preservation in KOA rats. Mid-term EA treatment only reduced pain, while delayed EA treatment resulted in no effects in both aspects. 1 mg of MIA produces steady pain behavior and progressive joint damage, which was suitable for EA treatment evaluation. Early-on EA treatment provided both joint protection and pain reduction, while Mid-term EA could only be used for studying EA-induced analgesia in KOA.
在临床实践中，针灸被广泛用于治疗膝关节骨关节炎（KOA）。在本研究中，我们旨在建立一种用于电针（EA）研究的标准KOA动物模型，并为进一步的KOA研究提供针灸配方。对关节内分别注射碘乙酸钠（MIA，0.3、1或3mg，每组n = 12）的大鼠进行疼痛样行为评估：爪退缩机械阈值、负重缺陷和关节病理变化（OARSI评分），直至损伤后28天。然后使用合适的剂量（1mg MIA），在3组（每组n = 16）中评估EA治疗（双侧ST36和ST35穴位，2/10Hz，30分钟/天，6天/周，共2周）的治疗效果：早期EA组、中期EA组和延迟EA组，其中EA分别在注射MIA后的第1天、第7天或第14天开始。1mg和3mg MIA均引起明显的关节损伤和持续的疼痛行为。但接受3mg MIA的动物在14天内迅速出现软骨和骨损伤。早期EA治疗可显著缓解KOA大鼠的疼痛并保护关节结构。中期EA治疗仅减轻疼痛，而延迟EA治疗在两方面均无效果。1mg MIA产生稳定的疼痛行为和进行性关节损伤，适合用于EA治疗评估。早期EA治疗既能保护关节又能减轻疼痛，而中期EA仅可用于研究KOA中EA诱导的镇痛作用。
