荧光相关光谱中的样本平移理论。
Theory of sample translation in fluorescence correlation spectroscopy.
作者信息
Palmer A G, Thompson N L
出版信息
Biophys J. 1987 Feb;51(2):339-43. doi: 10.1016/S0006-3495(87)83340-4.
Abstract
New applications of the technique of fluorescence correlation spectroscopy (FCS) require lateral translation of the sample through a focused laser beam (Peterson, N.O., D.C. Johnson, and M.J. Schlesinger, 1986, Biophys. J., 49:817-820). Here, the effect of sample translation on the shape of the FCS autocorrelation function is examined in general. It is found that if the lateral diffusion coefficients of the fluorescent species obey certain conditions, then the FCS autocorrelation function is a simple product of one function that depends only on transport coefficients and another function that depends only on the rate constants of chemical reactions that occur in the sample. This simple form should allow manageable data analyses in new FCS experiments that involve sample translation.
摘要
荧光相关光谱法（FCS）技术的新应用需要将样品横向平移通过聚焦激光束（Peterson, N.O., D.C. Johnson, and M.J. Schlesinger, 1986, Biophys. J., 49:817 - 820）。在此，我们全面研究了样品平移对FCS自相关函数形状的影响。结果发现，如果荧光物质的横向扩散系数满足某些条件，那么FCS自相关函数是一个仅取决于传输系数的函数与另一个仅取决于样品中发生的化学反应速率常数的函数的简单乘积。这种简单形式应有助于在涉及样品平移的新FCS实验中进行易于管理的数据分析。
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