Postgrado en Recursos Genéticos y Productividad - Fruticultura, Campus Montecillo, Colegio de Postgraduados, Texcoco, State of Mexico, Mexico.
Postgrado en Ciencias Forestales, Campus Montecillo, Colegio de Postgraduados, Texcoco, State of Mexico, Mexico.
Methods Mol Biol. 2024;2759:199-213. doi: 10.1007/978-1-0716-3654-1_19.
The linaloe [Bursera linanoe (La Llave) Rzed, Calderon and Medina] is an endemic species of Mexico, representative of the low deciduous forest of the states of Guerrero, Puebla, Morelos, and Oaxaca, and has been of great economic importance for the people, mainly for the artisanal use of its aromatic wood that is used to make boxes, trunks, and furniture that are manufactured in Olinala, Guerrero, Mexico; and industrial, thanks to the fine aroma of its essential oil (linalool), which is used in the manufacture of perfumes and pharmaceuticals. Overexploitation has endangered the species in recent years, and propagation by seed and/or cuttings has produced very poor results compared to those obtained with other recalcitrant Bursera species. The protection of endangered species makes urgent the need to propose new alternatives for its propagation. Somatic embryogenesis is a reliable and feasible technique, including induction, maintenance, multiplication, and maturation of embryos, often in semisolid culture media; however, the recent use of liquid media has allowed semi-automation in temporary immersion bioreactors, for example, the RITA system, which favors both the multiplication rate and the final conversion to seedlings.
龙舌兰科林纳罗（La Llave）Rzed、卡尔德隆和梅迪纳）是墨西哥特有的物种，代表格雷罗州、普埃布拉州、莫雷洛斯州和瓦哈卡州的落叶低地森林，对人民具有重要的经济意义，主要是因为其芳香木材的手工艺用途，用于制造盒子、箱子和家具，这些家具在墨西哥格雷罗州的奥纳拉制造；以及工业，因为其精油（芳樟醇）的香气细腻，用于制造香水和药品。近年来，过度开发已经危及到该物种，与其他抗逆性龙舌兰物种相比，种子和/或插条繁殖的效果非常差。保护濒危物种迫切需要提出新的繁殖替代方案。体细胞胚胎发生是一种可靠且可行的技术，包括胚胎的诱导、维持、增殖和成熟，通常在半固体培养基中进行；然而，最近在液体培养基中的使用允许在临时浸没生物反应器中进行半自动操作，例如 RITA 系统，这有利于增殖率和最终转化为幼苗。
