Center for Nuclear Energy in Agriculture - Plant Biotechnology Lab., University of São Paulo, Piracicaba, SP, Brazil.
Crop Science Departament - Tissue Culture and Ornamental Plants Lab., University of São Paulo, Piracicaba, SP, Brazil.
Methods Mol Biol. 2024;2759:157-164. doi: 10.1007/978-1-0716-3654-1_15.
The use of temporary immersion systems (TIS) for plant micropropagation is an efficient technique for plant production, and we have applied it for the production of alstroemerias. This method involves the cultivation of explants such as rhizomes and axillary buds in a nutrient medium to stimulate shoot growth. TIS offer advantages such as accelerated multiplication processes, uniform production, and cost reduction. This process has shown promise in meeting the growing demand for alstroemeria plants in the market. This chapter describes a specific protocol for temporary immersion bioreactor micropropagation of the "Albatroz" cultivar, with the potential for large-scale automation.
利用临时浸没系统（TIS）进行植物微繁殖是一种高效的植物生产技术，我们已经将其应用于满天星的生产。该方法涉及在营养培养基中培养根茎和腋芽等外植体，以刺激芽的生长。TIS 具有加速繁殖过程、均匀生产和降低成本等优点。该方法有望满足市场对满天星植物日益增长的需求。本章描述了一种特定的“Albatroz”品种的临时浸没生物反应器微繁殖方案，具有大规模自动化的潜力。
