Department of Internal Medicine I, University Hospital Tübingen, Otfried-Müller-Straße 10, 72076 Tübingen, Germany.
M3 Research Center for Malignome, Metabolome and Microbiome, Faculty of Medicine, University Tübingen, Otfried-Müller-Straße 37, 72076 Tübingen, Germany.
J Leukoc Biol. 2024 Mar 29;115(4):750-759. doi: 10.1093/jleuko/qiad167.
This study presents a high-dimensional immunohistochemistry approach to assess human γδ T cell subsets in their native tissue microenvironments at spatial resolution, a hitherto unmet scientific goal due to the lack of established antibodies and required technology. We report an integrated approach based on multiplexed imaging and bioinformatic analysis to identify γδ T cells, characterize their phenotypes, and analyze the composition of their microenvironment. Twenty-eight γδ T cell microenvironments were identified in tissue samples from fresh frozen human colon and colorectal cancer where interaction partners of the immune system, but also cancer cells were discovered in close proximity to γδ T cells, visualizing their potential contributions to cancer immunosurveillance. While this proof-of-principle study demonstrates the potential of this cutting-edge technology to assess γδ T cell heterogeneity and to investigate their microenvironment, future comprehensive studies are warranted to associate phenotypes and microenvironment profiles with features such as relevant clinical characteristics.
本研究提出了一种高维免疫组织化学方法，以评估人类 γδ T 细胞亚群在其天然组织微环境中的空间分辨率，由于缺乏已建立的抗体和所需的技术，这是一个迄今为止尚未实现的科学目标。我们报告了一种基于多重成像和生物信息学分析的综合方法，用于鉴定 γδ T 细胞，表征其表型，并分析其微环境的组成。在来自新鲜冷冻人结肠和结直肠癌的组织样本中鉴定了 28 个 γδ T 细胞微环境，在这些微环境中发现了免疫系统的相互作用伙伴，以及与 γδ T 细胞紧密相邻的癌细胞，这可视化了它们对癌症免疫监视的潜在贡献。虽然这项原理验证研究表明了这项前沿技术评估 γδ T 细胞异质性和研究其微环境的潜力，但需要进行未来的全面研究，将表型和微环境谱与相关的临床特征等特征相关联。
