Young W S, Mezey E, Siegel R E
Brain Res. 1986 Dec;387(3):231-41. doi: 10.1016/0169-328x(86)90029-x.
35S-labeled synthetic oligodeoxyribonucleotide probes were used to measure levels of vasopressin (VP) and oxytocin (OT) mRNAs in rat hypothalamus by quantitative in situ hybridization histochemistry (ISHH). VP and OT mRNA-containing cells were seen in the paraventricular (PVN) and supraoptic (SON) nuclei. VP mRNA was found to increase five-fold in the parvocellular region of the PVN after adrenalectomy while no changes occurred in magnocellular VP or OT mRNA levels. In the Brattleboro rat, VP mRNA levels were decreased and OT mRNA levels increased in the magnocellular regions. RNA species containing the VP introns were present at one fortieth of the level of processed VP mRNA in control rats. We also performed ISHH followed by immunohistochemistry on the same sections. We found that VP and its encoding mRNA were always located together as were OT-neurophysin and its encoding mRNA. In this study, we extend previous work by showing the characteristic distributions in the PVN and SON of VP and OT mRNA-containing cells and by measuring neuropeptide mRNA changes.
使用35S标记的合成寡脱氧核糖核苷酸探针，通过定量原位杂交组织化学（ISHH）来测量大鼠下丘脑加压素（VP）和催产素（OT）mRNA的水平。在室旁核（PVN）和视上核（SON）中可见含VP和OT mRNA的细胞。肾上腺切除术后，PVN小细胞区域的VP mRNA增加了五倍，而大细胞VP或OT mRNA水平没有变化。在布拉特洛维大鼠中，大细胞区域的VP mRNA水平降低，OT mRNA水平升高。含VP内含子的RNA种类在对照大鼠中的水平是加工后的VP mRNA水平的四十分之一。我们还在同一切片上先进行ISHH，然后进行免疫组织化学。我们发现VP及其编码mRNA总是位于一起，OT-神经垂体素及其编码mRNA也是如此。在本研究中，我们通过展示含VP和OT mRNA细胞在PVN和SON中的特征分布以及测量神经肽mRNA的变化，扩展了先前的工作。
