Ukaeje Onyebuchi C, Bandyopadhyay Bidhan C
Calcium Signaling Laboratory, Research Service, Veterans Affairs Medical Center, 50 Irving Street, NW, Washington, DC 20422, USA.
Crystals (Basel). 2024 Jan;14(1). doi: 10.3390/cryst14010011. Epub 2023 Dec 22.
The increased utilization of titanium dioxide (TiO) nanoparticles (TNPs) in various industrial and consumer products has raised concerns regarding its harmful effect due to its accumulation within the different systems of the human body. Here, we focused on the influence of TNPs on the growth and aggregation of two crucial crystalline substances, calcium phosphate (CaP) and monosodium urate (MSU), particularly its implications in gout disease. In this study, we adopted microscopic techniques and generated kinetic models to examine the interactions between TNPs, CaP and MSU, and crystallization, under controlled laboratory conditions. Our findings reveal that TNPs not only facilitate the growth of these crystals but also promote their co-aggregations. Crystal dissolution kinetics also exhibit that an increase in TNPs concentration corresponds to a reduction in the dissolution rate of CaP and MSU crystals in presence of the dissoluting agent hydroxycitrate (Hcit). These observations suggest that TNPs can stabilize CaP+MSU mixed crystals, which underscores the significance of TNPs' exposure in the pathogenesis of gout disease.
二氧化钛（TiO）纳米颗粒（TNPs）在各种工业和消费品中的使用增加，引发了人们对其在人体不同系统中积累所产生有害影响的担忧。在此，我们重点关注TNPs对两种关键晶体物质——磷酸钙（CaP）和尿酸钠（MSU）的生长和聚集的影响，特别是其在痛风疾病中的意义。在本研究中，我们采用微观技术并建立动力学模型，以在可控的实验室条件下研究TNPs、CaP和MSU之间的相互作用以及结晶情况。我们的研究结果表明，TNPs不仅促进这些晶体的生长，还促进它们的共聚集。晶体溶解动力学还显示，在存在溶解剂羟基柠檬酸（Hcit）的情况下，TNPs浓度的增加对应于CaP和MSU晶体溶解速率的降低。这些观察结果表明，TNPs可以稳定CaP + MSU混合晶体，这突出了TNPs暴露在痛风疾病发病机制中的重要性。
