青霉素过敏。通过酶联免疫吸附测定法测定和分类抗青霉素抗体。

Penicillin hypersensitivity. Determination and classification of anti-penicillin antibodies by the enzyme-linked immunosorbent assay.

作者信息

de Haan P, Boorsma D M, Kalsbeek G L

出版信息

Allergy. 1979 Apr 2;34(2):111-9. doi: 10.1111/j.1398-9995.1979.tb01371.x.

DOI:10.1111/j.1398-9995.1979.tb01371.x

Abstract

An enzyme-linked immunosorbent assay for the detection of anti-pencillin antibodies of the several Ig classes is described. The results of the ELISA in 350 sera of patients suspected of penicillin hypersensitivity are compared with those of the haemagglutination test. In 105 sera penicillin-specific IgM and/or IgG was demonstrated with the ELISA, the HA test being positive in 49 of these 105 sera. However , in another 14 sera IgM anti-penicillin antibodies could be shown only with the HA. In 10 sera penicillin-specific IgE was demonstrated with the ELISA, only four of these being also positive with the RAST. IgE was always found in combination with IgG and/or IgM. The positive correlation of the ELISA and the RAST with the intracutaneous test on penicillolypolylysine was 26.9% and 15.4% respectively. The ELISA is a simple and reproducible method for the detection of anti-penicillin antibodies, being more sensitive than the HA and the RAST.

摘要

本文描述了一种用于检测几种免疫球蛋白类抗青霉素抗体的酶联免疫吸附测定法。将350例疑似青霉素过敏患者血清的酶联免疫吸附测定（ELISA）结果与血凝试验结果进行了比较。ELISA检测出105份血清中有青霉素特异性IgM和/或IgG，这105份血清中有49份血凝试验呈阳性。然而，另有14份血清仅通过血凝试验显示出IgM抗青霉素抗体。ELISA检测出10份血清中有青霉素特异性IgE，其中只有4份放射性变应原吸附试验（RAST）也呈阳性。IgE总是与IgG和/或IgM同时存在。ELISA和RAST与青霉素多聚赖氨酸皮内试验呈阳性相关的比例分别为26.9%和15.4%。ELISA是一种检测抗青霉素抗体的简单且可重复的方法，比血凝试验和RAST更灵敏。