Rafiei Pouria, Raeeszadeh Mahdieh, Mohammadzadeh Peyman
Graduate of Faculty of Veterinary Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
Department of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
J Tissue Viability. 2024 Feb;33(1):135-143. doi: 10.1016/j.jtv.2024.01.003. Epub 2024 Jan 26.
Wound healing poses a challenging therapeutic scenario, requiring diverse clinical approaches.
This study aims to assess the wound-healing potential of Salix aegyptiaca's flower ointment compared to phenytoin, considering the active constituents of S. aegyptiaca and its traditional usage.
Initially, the active components of S. aegyptiaca were isolated and identified through the GC-MS technique. Subsequently, for the experimental intervention, thirty-five rats were divided into five distinct groups: control (C), phenytoin (F), and three S. aegyptiaca ointment groups at different concentrations (5 % - S5, 25 % - S25, and 50 % - S50). Anesthesia was administered, and wounds were induced on the animals' necks following a standard procedure. These wounds were then treated for a duration of 21 days. Wound healing progress was quantified, and histopathological assessments were conducted using hematoxylin and eosin staining and Mason's trichrome staining.
The main active compounds of S. aegyptiaca, namely n-hexadecanoic acid and oleic acid, were identified via GC-MS analysis. Although the initial group weights did not show a significant difference (P = 0.271), a significant variation was observed in the final weights (P = 0.003). The S50 group exhibited a lower wound healing rate than the S25 group on the 7th and 14th days but surpassed it on the 21st day (C < F < S5≈S25<S50). Pathological analysis of the S50 group revealed advanced re-epithelialization, heightened skin regeneration, increased angiogenesis, folliculogenesis, more orderly collagen fibers, and a marked reduction in cellular infiltration compared to the F group.
The findings highlight the superior wound-healing efficacy of 50 % S. aegyptiaca ointment over phenytoin ointment.
伤口愈合是一个具有挑战性的治疗场景，需要多种临床方法。
考虑到埃及柳的活性成分及其传统用途，本研究旨在评估埃及柳花软膏与苯妥英相比的伤口愈合潜力。
首先，通过气相色谱 - 质谱联用（GC - MS）技术分离和鉴定埃及柳的活性成分。随后，在实验干预中，将35只大鼠分为五个不同的组：对照组（C）、苯妥英组（F）以及三个不同浓度的埃及柳软膏组（5% - S5、25% - S25和50% - S50）。给予动物麻醉，并按照标准程序在其颈部诱导伤口。然后对这些伤口进行为期21天的治疗。对伤口愈合进展进行量化，并使用苏木精和伊红染色以及梅森三色染色进行组织病理学评估。
通过GC - MS分析鉴定出埃及柳的主要活性化合物，即正十六烷酸和油酸。虽然初始组体重没有显示出显著差异（P = 0.271），但最终体重观察到显著差异（P = 0.003）。S50组在第7天和第14天的伤口愈合率低于S25组，但在第21天超过了S25组（C < F < S5≈S25 < S50）。与F组相比，S50组的病理分析显示上皮再形成进展、皮肤再生增强、血管生成增加、毛囊形成、胶原纤维更有序，并且细胞浸润明显减少。
研究结果突出了50%埃及柳软膏比苯妥英软膏具有更好的伤口愈合疗效。
