Department of Biotechnology, Molecular Microbiology Laboratory, Indian Institute of Technology, Roorkee 247667, Uttarakhand, India.
Biomedical Sciences Research Institute, University of Ulster, Coleraine BT52 1SA, Northern Ireland, United Kingdom.
Biomed Pharmacother. 2017 Oct;94:1186-1196. doi: 10.1016/j.biopha.2017.08.010. Epub 2017 Aug 18.
A potent biosurfactant (BS) producing Bacillus licheniformis SV1 (NCBI GenBank Accession No. KX130852) was isolated from oil contaminated soil sample. Physicochemical investigations (TLC, HPLC, FTIR, GC-MS and NMR) revealed it to be glycolipid in nature. Fibroblast culture assay showed cytocompatibility and increased cell proliferation of 3T3/NIH fibroblast cells treated with this biosurfactant when checked using MTT assay and DAPI fluorescent staining. To evaluate the wound healing potential, BS ointment was formulated and checked for its spreadability and viscosity consistency. In vivo wound healing examination of full thickness skin excision wound rat model demonstrated the prompt re-epithelialization and fibroblast cell proliferation in the early phase while quicker collagen deposition in later phases of wound healing when BS ointment was used. These results validated the potential usage of BS ointment as a transdermal substitute for faster healing of impaired skin wound. Biochemical evaluation also substantiated the highest concentration of hydroxyproline (32.18±0.46, p<0.001) in the BS ointment treated animal tissue samples compared to the control. Hematoxylin-Eosin (H&E) and Masson's Trichrome staining validated the presence of increased amount of collagen fibers and blood vessels in the test animals treated with BS ointment.
从油污染土壤样本中分离出了一株产强效生物表面活性剂 (BS) 的地衣芽孢杆菌 SV1（NCBI GenBank 登录号 KX130852）。理化性质研究（TLC、HPLC、FTIR、GC-MS 和 NMR）表明，它是一种糖脂。成纤维细胞培养试验显示，用 MTT 法和 DAPI 荧光染色法检测到，用这种生物表面活性剂处理的 3T3/NIH 成纤维细胞具有细胞相容性和增殖能力。为了评估伤口愈合潜力，我们制备了 BS 软膏，并检查了其铺展性和粘度一致性。在全厚度皮肤切除创面大鼠模型的体内伤口愈合检查中，当使用 BS 软膏时，在早期阶段观察到迅速的再上皮化和成纤维细胞增殖，而在后期阶段更快地沉积胶原蛋白。这些结果验证了 BS 软膏作为一种经皮替代物，可加速受损皮肤伤口的愈合。生化评估还证实，与对照组相比，BS 软膏处理的动物组织样本中羟脯氨酸的浓度最高（32.18±0.46，p<0.001）。苏木精-伊红（H&E）和 Masson 三色染色验证了在接受 BS 软膏治疗的试验动物中存在更多的胶原蛋白纤维和血管。
