Botany and Microbiology Department, Faculty of Science, Damanhour University, Damanhour, 22511, Egypt.
Botany Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
Sci Rep. 2024 Jan 31;14(1):2547. doi: 10.1038/s41598-024-52982-9.
Over the past years, the assessment of myco-fabricated selenium nanoparticles (SeNPs) properties, is still in its infancy. Herein, we have highly stable myco-synthesized SeNPs using molecularly identified soil-isolated fungus; Penicillium tardochrysogenum OR059437; (PeSeNPs) were clarified via TEM, EDX, UV-Vis spectrophotometer, FTIR and zeta potential. The therapeutic efficacy profile will be determined, these crystalline PeSeNPs were examined for antioxidant, antimicrobial, MIC, and anticancer potentials, indicating that, PeSeNPs have antioxidant activity of (IC, 109.11 μg/mL) using DPPH free radical scavenging assay. Also, PeSeNPs possess antimicrobial potential against Penicillium italicum RCMB 001,018 (1) IMI 193,019, Methicillin-Resistant Staphylococcus aureus (MRSA) ATCC 4330 and Porphyromonas gingivalis RCMB 022,001 (1) EMCC 1699; with I.Z. diameters and MIC; 16 ± 0.5 mm and MIC 500 µg/ml, 11.9 ± 0.6 mm, 500 µg/ml and 15.9±0.6 mm, 1000 µg/ml, respectively. Additionally, TEM micrographs were taken for P. italicum treated with PeSeNPs, demonstrating the destruction of hyphal membrane and internal organelles integrity, pores formation, and cell death. PeSeNP alone in vivo and combined with a near-infrared physiotherapy lamp with an energy intensity of 140 mW/cm showed a strong therapeutic effect against cancer cells. Thus, PeSeNPs represent anticancer agents and a suitable photothermal option for treating different kinds of cancer cells with lower toxicity and higher efficiency than normal cells. The combination therapy showed a very large and significant reduction in tumor volume, the tumor cells showed large necrosis, shrank, and disappeared. There was also improvement in liver ultrastructure, liver enzymes, and histology, as well as renal function, urea, and creatinine.
在过去的几年中，对真菌合成的硒纳米粒子（SeNPs）性质的评估仍处于起步阶段。在这里，我们使用分子鉴定的土壤分离真菌 - 迟缓青霉 OR059437；（PeSeNPs）合成了高度稳定的真菌合成硒纳米粒子；通过 TEM、EDX、紫外可见分光光度计、FTIR 和 ζ 电位进行了澄清。将确定治疗效果概况，这些结晶性 PeSeNPs 被检查抗氧化、抗菌、MIC 和抗癌潜力，表明，PeSeNPs 在 DPPH 自由基清除测定中具有抗氧化活性（IC，109.11μg/mL）。此外，PeSeNPs 对青霉属意大利 RCMB 001、018（1）IMI 193、耐甲氧西林金黄色葡萄球菌（MRSA）ATCC 4330 和牙龈卟啉单胞菌 RCMB 022、001（1）EMCC 1699 具有抗菌潜力；具有 I.Z.直径和 MIC；16±0.5mm 和 MIC500μg/ml、11.9±0.6mm、500μg/ml 和 15.9±0.6mm、1000μg/ml，分别。此外，对用 PeSeNPs 处理的青霉属意大利拍摄了 TEM 显微照片，证明了菌丝膜和内部细胞器完整性的破坏、孔形成和细胞死亡。PeSeNP 单独在体内并与能量强度为 140mW/cm 的近红外光疗灯结合使用，对癌细胞具有很强的治疗作用。因此，PeSeNPs 代表抗癌剂和合适的光热选择，用于治疗不同类型的癌细胞，其毒性低于正常细胞，效率更高。联合治疗显示肿瘤体积非常大且显著减少，肿瘤细胞显示大坏死、收缩和消失。肝脏超微结构、肝功能、组织学以及肾功能、尿素和肌酐也得到了改善。
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