Department of Botany, PMAS-Arid-Agriculture University Rawalpindi, Rawalpindi 46000, Pakistan.
Department of Botany, University of Poonch Rawalakot, Rawalakot 12350, Pakistan.
Molecules. 2022 Aug 15;27(16):5194. doi: 10.3390/molecules27165194.
Nanotechnology, the science of the recent era, has diverse applications in agriculture. Selenium (Se) is a non-metal and an essential micronutrient for animals and humans. In this study, selenium nanoparticles (SeNPs) were biosynthesized by using fruit extracts. The size, shape, chemical nature, and identification of functional groups involved in the synthesis of SeNPs were studied by UV-visible spectroscopy, Scanning Electron Microscope (SEM), and Fourier Transform Infra-Red (FTIR) spectrometry. SeNP synthesis was confirmed by an absorption peak at 258 nm by UV-visible spectroscopy. SEM showed that SeNPs were spherical, smooth, and between 60 and 80 nm in size. FTIR spectrometry confirmed the presence of terpenes, alcohols, ketones, aldehydes, and esters as well as phyto-constituents, such as alkaloids and flavonoids, that possibly act as reducing or capping agents of SeNPs in an aqueous solution of . Antimicrobial activity was examined against bacterial pathogens, such as , , , and , as well as fungal pathogens, such as and , by using the well-diffusion method. Antioxidant activity was observed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, ABTs assay, and reducing power assay. At a higher concentration of 400 ppm, biosynthesized SeNPs showed an inhibition zone of 20.5 mm, 20 mm, 21 mm, and 18.5 mm against , , , and Staphylococcus epidermitis, respectively. Similarly, SeNPs also demonstrated a zone of inhibition against and of 17.5 and 21 mm, respectively. In contrast to fruit extracts, -mediated SeNPs demonstrated strong antimicrobial activity. By performing the DPPH, ABTs, and reducing power assay, SeNPs showed 85.2 ± 0.009, 81.12 ± 0.007, and 80.37 ± 0.0035% radical scavenging potential, respectively. The present study could contribute to the drug development and nutraceutical industries.
纳米技术是当今时代的科学，在农业中有多种应用。硒（Se）是动物和人类必需的微量元素。在这项研究中，使用水果提取物生物合成了硒纳米粒子（SeNPs）。通过紫外-可见光谱、扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）研究了 SeNPs 的尺寸、形状、化学性质和参与合成的功能基团的鉴定。紫外-可见光谱的吸收峰在 258nm 处证实了 SeNP 的合成。SEM 显示，SeNPs 为球形，光滑，大小在 60-80nm 之间。FTIR 光谱证实了萜类、醇类、酮类、醛类和酯类以及植物成分（如生物碱和类黄酮）的存在，这些成分可能在水溶液中作为 SeNPs 的还原剂或封端剂。通过琼脂扩散法，检测了生物合成的 SeNPs 对细菌病原体（如 、 、 、 和 ）和真菌病原体（如 和 ）的抗菌活性。抗氧化活性通过 2,2-二苯基-1-苦基肼（DPPH）测定法、ABTs 测定法和还原力测定法进行观察。在较高浓度 400ppm 下，生物合成的 SeNPs 对 、 、 和表皮葡萄球菌的抑制圈分别为 20.5mm、20mm、21mm 和 18.5mm。同样，SeNPs 对 和 的抑制圈分别为 17.5mm 和 21mm。与 水果提取物相比，-介导的 SeNPs 表现出较强的抗菌活性。通过 DPPH、ABTs 和还原力测定法，SeNPs 表现出 85.2±0.009%、81.12±0.007%和 80.37±0.0035%的自由基清除能力。本研究可能有助于药物开发和营养保健品行业。
