Febriansah Rifki, Hertiani Triana, Widada Jaka, Taher Muhammad, Damayanti Ema, Mustofa Mustofa
School of Pharmacy, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Yogyakarta, Indonesia.
Pharmacognosy and Phytochemistry Laboratory, Pharmaceutical Biology Department, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281.
Heliyon. 2024 Jan 5;10(2):e24195. doi: 10.1016/j.heliyon.2024.e24195. eCollection 2024 Jan 30.
The occurrence of resistance to anticancer and the emergence of serious side effects due to chemotherapy is one of the main problems in cancer treatment, including breast cancer. The need for effective anticancer with a specific target is urgently required. are widely known as the potential producers of new anticancer molecules. Previously reported that the methanol extract of GMY01 isolated from Krakal Coast, Gunungkidul had very strong cytotoxic activity against MCF-7 and T47D breast cancer cells with IC values of 0.6 and 1.3 μg/mL, respectively. The following study aimed to isolate and identify active compounds of the GMY01 and evaluate its cytotoxic activity. The study was started by re-culturing and re-fermented optimization of GMY01 in a larger volume, then the bacteria were extracted using methanol following the bioassay-guided isolation of the extract obtained. The active compounds obtained were then structurally determined using UV/Vis spectroscopy, Fourier Transform-Infrared (FT-IR), Liquid Chromatography-Mass Spectroscopy (LC-MS), H NMR, and C NMR and analyzed for their cytotoxic activity using MTT assay on MCF-7 and normal Vero cells line. The results showed that the culture of the GMY01 using Starch Nitrate Broth (SNB) media yields the best results compared to other culture media. An active anticancer compound namely mannotriose was successfully isolated from the methanol extract with an IC value of 5.6 μg/mL and 687 μg/mL against the MCF-7 and Vero cells lines, respectively, indicating that this compound showed strong cytotoxic activity with high selectivity.
抗癌药物耐药性的出现以及化疗导致的严重副作用的产生是癌症治疗（包括乳腺癌治疗）中的主要问题之一。迫切需要具有特定靶点的有效抗癌药物。众所周知，[此处原文可能有误，推测为某些微生物等]是新抗癌分子的潜在生产者。此前报道，从Gunungkidul的Krakal海岸分离出的GMY01的甲醇提取物对MCF - 7和T47D乳腺癌细胞具有非常强的细胞毒性活性，IC值分别为0.6和1.3μg/mL。以下研究旨在分离和鉴定GMY01的活性化合物并评估其细胞毒性活性。该研究首先对GMY01进行更大体积的再培养和再发酵优化，然后按照生物测定指导的提取物分离方法，用甲醇提取细菌。然后使用紫外/可见光谱、傅里叶变换红外光谱（FT - IR）、液相色谱 - 质谱（LC - MS）、1H NMR和13C NMR对获得的活性化合物进行结构测定，并使用MTT法在MCF - 7和正常Vero细胞系上分析其细胞毒性活性。结果表明，与其他培养基相比，使用硝酸淀粉肉汤（SNB）培养基培养GMY01产生的效果最佳。从甲醇提取物中成功分离出一种活性抗癌化合物甘露三糖，其对MCF - 7和Vero细胞系的IC值分别为5.6μg/mL和687μg/mL，表明该化合物具有很强的细胞毒性活性和高选择性。
