从小桐子根中提取新型多糖的纯化、结构表征及抗癌活性研究。
Purification, structural characterization and anticancer activity of the novel polysaccharides from Rhynchosia minima root.
机构信息
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao, China.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
出版信息
Carbohydr Polym. 2015 Nov 5;132:67-71. doi: 10.1016/j.carbpol.2015.05.059. Epub 2015 Jun 4.
Three novel acidic polysaccharides termed PRM1, PRM3 and PRM5 were purified from Rhynchosia minima root using DEAE-52 cellulose and sephadex G-150 column chromatography. Their structures were characterized by ultraviolet (UV) and Fourier transform infrared (FTIR) spectrometry, gel permeation chromatography (GPC), gas chromatography-mass spectrometry (GC-MS), and differential scanning colorimeter (DSC) analysis. The uronic acid contents of PRM1, PRM3 and PRM5 were 30.7%, 12.7% and 47.7%, respectively. PRM1 (143.2 kDa), PRM3 (105.3 kDa) and PRM5 (162.1 kDa) were heteropolysaccharides because they were composed of arabinose, mannose, glucose and galactose. Their enthalpy values were 201.0, 111.0 and 206.8 J/g, respectively. PRM3 and PRM1 exhibited strong in vitro anticancer activity against lung cancer A549 and liver cancer HepG2 cells in a dose-dependent manner. These findings suggested that PRM1 and PRM3 could be potentially developed as natural anticancer agents.
从山黧豆根中分离得到 3 种新型酸性多糖 PRM1、PRM3 和 PRM5，采用 DEAE-52 纤维素和葡聚糖凝胶 G-150 柱层析对其进行分离纯化。通过紫外（UV）和傅里叶变换红外（FTIR）光谱、凝胶渗透色谱（GPC）、气相色谱-质谱联用（GC-MS）和差示扫描量热仪（DSC）分析对其结构进行表征。PRM1、PRM3 和 PRM5 的糖醛酸含量分别为 30.7%、12.7%和 47.7%。PRM1（143.2 kDa）、PRM3（105.3 kDa）和 PRM5（162.1 kDa）是杂多糖，因为它们由阿拉伯糖、甘露糖、葡萄糖和半乳糖组成。它们的焓值分别为 201.0、111.0 和 206.8 J/g。PRM3 和 PRM1 对肺癌 A549 和肝癌 HepG2 细胞表现出较强的体外抗癌活性，呈剂量依赖性。这些发现表明 PRM1 和 PRM3 可能被开发为天然抗癌药物。
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