State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao, China.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Carbohydr Polym. 2015 Nov 5;132:67-71. doi: 10.1016/j.carbpol.2015.05.059. Epub 2015 Jun 4.
Three novel acidic polysaccharides termed PRM1, PRM3 and PRM5 were purified from Rhynchosia minima root using DEAE-52 cellulose and sephadex G-150 column chromatography. Their structures were characterized by ultraviolet (UV) and Fourier transform infrared (FTIR) spectrometry, gel permeation chromatography (GPC), gas chromatography-mass spectrometry (GC-MS), and differential scanning colorimeter (DSC) analysis. The uronic acid contents of PRM1, PRM3 and PRM5 were 30.7%, 12.7% and 47.7%, respectively. PRM1 (143.2 kDa), PRM3 (105.3 kDa) and PRM5 (162.1 kDa) were heteropolysaccharides because they were composed of arabinose, mannose, glucose and galactose. Their enthalpy values were 201.0, 111.0 and 206.8 J/g, respectively. PRM3 and PRM1 exhibited strong in vitro anticancer activity against lung cancer A549 and liver cancer HepG2 cells in a dose-dependent manner. These findings suggested that PRM1 and PRM3 could be potentially developed as natural anticancer agents.
从山黧豆根中分离得到 3 种新型酸性多糖 PRM1、PRM3 和 PRM5，采用 DEAE-52 纤维素和葡聚糖凝胶 G-150 柱层析对其进行分离纯化。通过紫外（UV）和傅里叶变换红外（FTIR）光谱、凝胶渗透色谱（GPC）、气相色谱-质谱联用（GC-MS）和差示扫描量热仪（DSC）分析对其结构进行表征。PRM1、PRM3 和 PRM5 的糖醛酸含量分别为 30.7%、12.7%和 47.7%。PRM1（143.2 kDa）、PRM3（105.3 kDa）和 PRM5（162.1 kDa）是杂多糖，因为它们由阿拉伯糖、甘露糖、葡萄糖和半乳糖组成。它们的焓值分别为 201.0、111.0 和 206.8 J/g。PRM3 和 PRM1 对肺癌 A549 和肝癌 HepG2 细胞表现出较强的体外抗癌活性，呈剂量依赖性。这些发现表明 PRM1 和 PRM3 可能被开发为天然抗癌药物。
