Hugh D, Grennan A, Abugila M A, Weinkove C
Clin Chem. 1987 Apr;33(4):569-71.
Sodium metabisulfite, commonly used to prevent the oxidation of catecholamines during extraction from plasma onto alkaline alumina, does not prevent their subsequent degradation in acetic acid eluates. However, ascorbic acid, a potent antioxidant, is extracted with the catecholamines onto the alumina and prevents such destruction. However, ascorbic acid may interfere with the electrochemical measurement of catecholamines, unless sequential oxidation and reduction are used. Other methods of minimizing catecholamine oxidation in acetic acid eluates include refrigerating at 4 degrees C and capping the sample vials to exclude atmospheric oxygen.
焦亚硫酸钠常用于防止从血浆中提取到碱性氧化铝上的儿茶酚胺氧化，但不能防止其在乙酸洗脱液中随后的降解。然而，抗坏血酸作为一种有效的抗氧化剂，会与儿茶酚胺一起被提取到氧化铝上，并防止这种破坏。然而，抗坏血酸可能会干扰儿茶酚胺的电化学测量，除非采用顺序氧化和还原的方法。在乙酸洗脱液中使儿茶酚胺氧化最小化的其他方法包括在4℃下冷藏以及密封样品瓶以排除大气中的氧气。
