Kaufmann A, Uhlhaas S, Friedl W, Propping P
Clin Chim Acta. 1987 Jan 30;162(2):215-9. doi: 10.1016/0009-8981(87)90453-0.
Using isoelectric focusing of human erythrocyte transketolase, the isoenzyme pattern described recently (Kaczmarek and Nixon, 1983) was reexamined. Seven bands having pI values of 7.4-8.4 were common to the central part of the transketolase isoenzyme pattern in 63 healthy subjects investigated and were definitely reproduced, whereas four additional marginal bands (pI values of 7.2, 7.3, 8.6 and 8.8) were found with varying intensities in part of the samples and could not always be reproduced. We conclude that the method used does not permit the distinction of transketolase variants, that would allow to postulate a genetic polymorphism, based only on variation of the marginal bands of the pattern.
利用人红细胞转酮醇酶的等电聚焦技术，对最近描述的同工酶模式（Kaczmarek和Nixon，1983年）进行了重新研究。在63名接受调查的健康受试者中，转酮醇酶同工酶模式中央部分共有七条pI值为7.4 - 8.4的条带，且能明确重现，而另外四条边缘条带（pI值为7.2、7.3、8.6和8.8）在部分样本中强度各异，并非总能重现。我们得出结论，仅基于模式边缘条带的变化，所采用的方法无法区分可用于假定遗传多态性的转酮醇酶变体。
