Kaczmarek M J, Nixon P F
Clin Chim Acta. 1983 Jun 15;130(3):349-56. doi: 10.1016/0009-8981(83)90309-1.
Analytical isoelectric focusing and a stain for transketolase have been applied to partially purified samples of human erythrocyte hemolysates and have detected individual species of transketolase having pI values of 6.6, 7.3, 7.5, 7.8, 8.1, 8.2, 8.4 and 9.2. Six different patterns of these species were detected in 25 healthy subjects. The species of pI 7.5, 7.8 and 8.1 were common to all six patterns. Species isolated by electrofocusing could be rerun in the same system with identical pI value. The addition of thiamin diphosphate to the staining mixture darkened some but not all bands of transketolase activity. Thus human erythrocyte transketolase is heterogeneous and appears to share with human fibroblast transketolase heterogeneity for affinity of the cofactor. This heterogeneity might need recognition when thiamin nutritional sufficiency is assessed by the 'thiamin diphosphate effect' on erythrocyte transketolase.
分析等电聚焦法和转酮醇酶染色法已应用于部分纯化的人红细胞溶血产物样本，并检测到转酮醇酶的各个种类，其等电点（pI）值分别为6.6、7.3、7.5、7.8、8.1、8.2、8.4和9.2。在25名健康受试者中检测到这些种类的六种不同模式。pI为7.5、7.8和8.1的种类在所有六种模式中都存在。通过电聚焦分离出的种类可以在同一系统中重新运行，且pI值相同。在染色混合物中添加硫胺二磷酸会使转酮醇酶活性的一些但不是所有条带变深。因此，人红细胞转酮醇酶是异质性的，并且在辅因子亲和力方面似乎与人成纤维细胞转酮醇酶具有相同的异质性。当通过对红细胞转酮醇酶的“硫胺二磷酸效应”评估硫胺营养充足性时，可能需要认识到这种异质性。
