Department of Neurology, People's Hospital of Dongxihu District.
Department of Urology, People's Hospital of Dongxihu District.
Tohoku J Exp Med. 2024 May 30;263(1):55-62. doi: 10.1620/tjem.2024.J007. Epub 2024 Mar 1.
Depression disorder has become a major mental disease and has attracted special attention globally. Identifying specific biomarkers for the diagnosis and severity of depression disorder would benefit its clinical management. This study focused on the significance of lncRNA SNHG14 in depression disorder and investigated its effect on depression-like behaviors, aiming to explore a potential biomarker for depression disorder occurrence and development. This study included 147 patients with depression disorder and 98 healthy individuals. The serum SNHG14 in all participants was analyzed by PCR, and its diagnostic value was evaluated by receiver operatorating characteristic curve (ROC) analysis. The depression-like behaviors were induced via chronic social defeat stress (CSDS) and evaluated by sucrose preference, forced swimming, and open field tests. SNHG14 was significantly upregulated in depression disorder patients relative to healthy individuals, which discriminated depression disorder patients with a relatively high efficiency. Depression disorder patients with severe conditions showed higher serum SNHG14 levels, and a significantly positive correlation of SNHG14 with PHQ9 score was demonstrated. In CSDS mice, increasing SNHG14 and decreasing miR-200a-3p were observed. Silencing SNHG14 and overexpressing miR-200a-3p could alleviate reduced sucrose preference, increased swimming immobility time, decreased standing times, and decreased traveling distance induced by CSDS. The knockdown of SNHG14 promoted the expression of miR-200a-3p, and silencing miR-200a-3p could reverse the protective effect of SNHG14 silencing on depression-like behaviors. SNHG14 served as a biomarker for the occurrence and severity of depression disorder. Silencing SNHG14could alleviate depression-like behaviors via modulating miR-200a-3p.
抑郁症已成为一种主要的精神疾病，在全球范围内受到特别关注。确定抑郁症的诊断和严重程度的特定生物标志物将有益于其临床管理。本研究关注 lncRNA SNHG14 在抑郁症中的意义，并研究其对抑郁样行为的影响，旨在探索抑郁症发生和发展的潜在生物标志物。本研究纳入了 147 例抑郁症患者和 98 例健康个体。采用 PCR 分析所有参与者的血清 SNHG14，并通过接受者操作特征曲线（ROC）分析评估其诊断价值。采用慢性社会挫败应激（CSDS）诱导抑郁样行为，并通过蔗糖偏好、强迫游泳和旷场试验进行评估。与健康个体相比，抑郁症患者的血清 SNHG14 显著上调，且对抑郁症患者的区分效率较高。病情严重的抑郁症患者血清 SNHG14 水平较高，且 SNHG14 与 PHQ9 评分呈显著正相关。在 CSDS 小鼠中，观察到 SNHG14 增加和 miR-200a-3p 减少。沉默 SNHG14 和过表达 miR-200a-3p 可减轻 CSDS 引起的蔗糖偏好减少、游泳不动时间增加、站立时间减少和行进距离减少。SNHG14 的敲低促进了 miR-200a-3p 的表达，沉默 miR-200a-3p 可逆转 SNHG14 沉默对抑郁样行为的保护作用。SNHG14 是抑郁症发生和严重程度的生物标志物。沉默 SNHG14 可通过调节 miR-200a-3p 减轻抑郁样行为。
