Harti Shreya A, Adarsha M S, Meena N, Priya N S, Vijayalakshmi L, Airsang Akshata J
Department of Conservative Dentistry and Endodontics, Private Practitioner, Davangere, Karnataka, India.
Department of Conservative Dentistry and Endodontics, Vokkaligara Sangha Dental College and Hospital, Bengaluru, Karnataka, India.
J Oral Maxillofac Pathol. 2023 Oct-Dec;27(4):700-705. doi: 10.4103/jomfp.jomfp_467_23. Epub 2023 Dec 20.
The success of the root-end procedure depends on the regeneration of the functional periodontal attachment system, including the cementum on the resected root-end surface, periodontal ligament (PDL), and alveolar bone. As root end filling materials remain in close contact with live periapical tissues, they may influence the endodontic treatment outcome.
To assess and compare the cytotoxicity and genotoxicity of three root repair materials, mineral trioxide aggregate (MTA), endosequence, and geristore in human-cultured periodontal ligament fibroblasts.
Cultured human periodontal ligament fibroblasts of the third passage were used in the study. They were placed in contact with the root repair materials. The cytotoxic effect on PDL fibroblasts was determined by (3-(4,5-dimethylthiazol-2-yl)-2,5-tetrazolium bromide) assay after 24 hours and 48 hours intervals. Cell viability was determined using an inverted phase contrast microscope. The genotoxic effect on the periodontal fibroblast cells was determined by comet assay using imaging software.
Data were analyzed using Tukey's multiple comparison test and Dunnett's multiple test.
All the test materials showed higher cytotoxicity and genotoxicity at the 48 hour interval with a statistically significant difference from the control group (P < 0.05). MTA was shown to be least cytotoxic and genotoxic to PDL fibroblasts, followed by endosequence root repair material and geristore at 24 hour and 48 hour intervals.
The cytotoxicity and genotoxicity of MTA were the least compared to endosequence and geristore on human-cultured PDL fibroblasts.
根尖手术的成功取决于功能性牙周附着系统的再生，包括切除的根尖表面的牙骨质、牙周膜（PDL）和牙槽骨。由于根尖充填材料与活的根尖周组织密切接触，它们可能会影响牙髓治疗的结果。
评估和比较三种根尖修复材料，即矿物三氧化物凝聚体（MTA）、Endosequence和Geristore对人培养的牙周膜成纤维细胞的细胞毒性和遗传毒性。
研究使用第三代培养的人牙周膜成纤维细胞。将它们与根尖修复材料接触。在24小时和48小时间隔后，通过（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐）试验测定对PDL成纤维细胞的细胞毒性作用。使用倒置相差显微镜测定细胞活力。使用成像软件通过彗星试验测定对牙周成纤维细胞的遗传毒性作用。
使用Tukey多重比较检验和Dunnett多重检验分析数据。
所有测试材料在48小时间隔时均显示出较高的细胞毒性和遗传毒性，与对照组相比有统计学显著差异（P < 0.05）。在24小时和48小时间隔时，MTA对PDL成纤维细胞的细胞毒性和遗传毒性最小，其次是Endosequence根尖修复材料和Geristore。
在人培养的PDL成纤维细胞上，与Endosequence和Geristore相比，MTA的细胞毒性和遗传毒性最小。
