Samyuktha Voruganti, Ravikumar Pabbati, Nagesh Bolla, Ranganathan K, Jayaprakash Thumu, Sayesh Vemuri
Department of Conservative Dentistry and Endodontics, SIBAR Institute of Dental Sciences, Guntur, Andhra Pradesh, India.
Department of Oral and Maxillofacial Pathology, RAGAS Dental College, Chennai, Tamil Nadu, India.
J Conserv Dent. 2014 Sep;17(5):467-70. doi: 10.4103/0972-0707.139844.
To evaluate the cytotoxicity of three root repair materials, mineral trioxide aggregate (MTA), Endosequence Root Repair Material and Biodentine in human periodontal ligament fibroblasts.
Periodontal ligament fibroblasts were cultured from healthy premolar extracted for orthodontic purpose. Cells in the third passage were used in the study. The cultured fibroblast cells were placed in contact with root repair materials: (a) Biodentine, (b) MTA, (c) Endosequence, (d) control. The effects of these three materials on the viability of Periodontal ligament (PDL) fibroblasts were determined by trypan blue dye assay after 24 hours and 48-hour time period. Cell viability was determined using inverted phase contrast microscope.
Cell viability was compared for all the experimental groups with Wilcoxons matched pair test.
At the 24-hour examination period, all the materials showed increased cell viability. At 48-hour time period, there is slight decrease in cell viability. Mineral trioxide aggregate showed statistically significant increase in the cell viability when compared to other root repair materials.
Mineral trioxide aggregate was shown to be less toxic to periodontal ligament fibroblasts than Endosequence Root Repair Material and Biodentine.
评估三种根管修复材料，即三氧化矿物凝聚体（MTA）、Endosequence根管修复材料和生物陶瓷在人牙周膜成纤维细胞中的细胞毒性。
从因正畸目的拔除的健康前磨牙中培养牙周膜成纤维细胞。研究使用第三代细胞。将培养的成纤维细胞与根管修复材料接触：（a）生物陶瓷，（b）MTA，（c）Endosequence，（d）对照。在24小时和48小时时间段后，通过台盼蓝染色法测定这三种材料对牙周膜（PDL）成纤维细胞活力的影响。使用倒置相差显微镜测定细胞活力。
使用Wilcoxons配对检验比较所有实验组的细胞活力。
在24小时检查期，所有材料均显示细胞活力增加。在48小时时间段，细胞活力略有下降。与其他根管修复材料相比，三氧化矿物凝聚体显示细胞活力有统计学显著增加。
三氧化矿物凝聚体对牙周膜成纤维细胞的毒性低于Endosequence根管修复材料和生物陶瓷。
