Acharya Sonu, Gurunathan Deepa, Sahoo Dipmalla, Singh Bismay, Sahoo Alkananda, Acharya Sheetal
Department of Pediatric and Preventive Dentistry, Institute of Dental Sciences, Siksha'o'Anusandhan (Deemed to be) University, Bhubaneswar, Odisha, India.
Department of Pedodontics and Preventive Dentistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India.
J Int Soc Prev Community Dent. 2023 Dec 27;13(6):493-499. doi: 10.4103/jispcd.JISPCD_68_23. eCollection 2023 Nov-Dec.
Mineral trioxide aggregate (MTA) is a relatively new versatile dental material. MTA has many advantages as well as disadvantages. To reduce most of the drawbacks of MTA, a premixed bioceramic MTA, NeoPutty MTA, was introduced in 2020. In this study, we assessed the antimicrobial activity of the newer MTA, NeoPutty MTA. We modified NeoPutty MTA and compared both against , , , and .
Using the agar diffusion method, NeoPutty MTA was tested for antibacterial activity against the above-mentioned microorganisms. A base layer of Petri plates was done using Muller-Hinton agar for , , and and brain heart infusion agar for . A total of 32 plates were employed; the plates were divided randomly into four test groups having eight plates each, so microorganisms were tested eight times. Three cavities were made in agar and filled with freshly mixed materials after 24 h. A pour plate seeded the microorganisms. The plates were pre-incubated for 2 h at room temperature and incubated at 37°C for 24 h. An independent observer measured the inhibition zone diameters.
NeoPutty MTA, when tested alone, did not show much antibacterial activity against , , and but had significant antimicrobial activity against when used at different concentrations. Modified NeoPutty (NeoPutty with antibiotics added individually) showed significant antibacterial activity against these microorganisms, as seen by the zone of inhibition of these bacteria.
Modified NeoPutty with antibiotics has a better antimicrobial effect than NeoPutty MTA.
三氧化矿物凝聚体（MTA）是一种相对较新的多功能牙科材料。MTA有许多优点也有缺点。为了减少MTA的大部分缺点，一种预混生物陶瓷MTA，即新型MTA（NeoPutty MTA）于2020年推出。在本研究中，我们评估了新型MTA（NeoPutty MTA）的抗菌活性。我们对新型MTA（NeoPutty MTA）进行了改良，并将两者与[此处原文缺失具体比较对象]进行了比较。
采用琼脂扩散法，对新型MTA（NeoPutty MTA）针对上述微生物的抗菌活性进行测试。对于[此处原文缺失具体微生物名称]，使用穆勒-欣顿琼脂制作培养皿的底层；对于[此处原文缺失具体微生物名称]，使用脑心浸液琼脂制作培养皿的底层。总共使用了32个培养皿；将培养皿随机分为四个测试组，每组八个培养皿，因此对微生物进行了八次测试。在琼脂中制作三个腔，24小时后用新鲜混合的材料填充。倾注平板接种微生物。平板在室温下预孵育2小时，然后在37°C下孵育24小时。由一名独立观察者测量抑菌圈直径。
单独测试时，新型MTA（NeoPutty MTA）对[此处原文缺失具体微生物名称]、[此处原文缺失具体微生物名称]和[此处原文缺失具体微生物名称]没有显示出太多抗菌活性，但在不同浓度下使用时对[此处原文缺失具体微生物名称]具有显著的抗菌活性。改良型新型MTA（分别添加抗生素的新型MTA）对这些微生物显示出显著的抗菌活性，从这些细菌的抑菌圈可以看出。
添加抗生素的改良型新型MTA比新型MTA（NeoPutty MTA）具有更好的抗菌效果。
