antimicrobial activity of mineral trioxide aggregate, Biodentine, and calcium-enriched mixture cement against , , and using the agar diffusion technique.

BACKGROUND

This study assessed the antimicrobial activity of Biodentine, mineral trioxide aggregate (MTA), and calcium-enriched mixture (CEM) cement against , , and .

MATERIALS AND METHODS

In this study, microbial suspensions were inoculated onto agar plates. The antimicrobial effects of MTA, Biodentine and CEM cement were assessed against , , and by the agar diffusion test. In each experimental group, 7 plates containing 3 wells were prepared and immediately filled with freshly mixed cements. Positive and negative control plates were prepared with/without the bacterial suspension, respectively. After 2 h of preincubation at room temperature, the plates were incubated at 37°C for 24 h. The diameter of growth inhibition zones was measured after 24 h. Data were analyzed using ANOVA and Tukey's test (α = 0.05).

RESULTS

Biodentine showed strong antimicrobial activity against all three microorganisms with an average inhibition zone of 9.10 mm. The inhibitory effect of Biodentine on and was significantly superior to that of the other two cements ( < 0.05). MTA and CEM cement showed significantly higher antimicrobial activity against ( < 0.05). The antimicrobial effects of Biodentine on and were significantly greater than on ( < 0.05).

CONCLUSION

All cements revealed antimicrobial properties against the tested microbial strains. Biodentine had stronger antimicrobial effects against and compared with MTA and CEM cement. Furthermore, the largest inhibition zones around all three cements belonged to .