某些猪胃蛋白酶和人胃蛋白酶对合成肽底物的特异性。
The specificity of some pig and human pepsins towards synthetic peptide substrates.
作者信息
Ryle A P, Auffret C A
出版信息
Biochem J. 1979 Apr 1;179(1):247-9. doi: 10.1042/bj1790247.
Abstract
- The peptidase activities of pig pepsins A and C and human pepsin and gastricsin were compared. 2. The peptides studied had the general formula A Leu Val-His-B. Hydrolysis at 37 degrees C and pH 2.07 occurred at the amino side of the leucine residue for all the enzymes and all the peptides. 3. When A was Ac-Ala the peptides were hydrolysed under these conditions slowly by pig pepsin C only. 4. Pig pepsin A and human pepsin were unable to hydrolyse the tyrosine-containing peptides under the conditions tested. Gastricsin (human pepsin C) had about one-third of the activity of pig pepsin C with these substrates. 5. The increase in the rate of hydrolysis caused by the extension of the chain by a single alanine residue was most marked for pig pepsin A and human pepsin.
摘要
- 对猪胃蛋白酶A和C、人胃蛋白酶和胃泌素的肽酶活性进行了比较。2. 所研究的肽具有通式A亮氨酸缬氨酸-组氨酸-B。在37℃和pH 2.07条件下，所有酶和所有肽的水解均发生在亮氨酸残基的氨基侧。3. 当A为乙酰丙氨酸时，在这些条件下，这些肽仅被猪胃蛋白酶C缓慢水解。4. 在测试条件下，猪胃蛋白酶A和人胃蛋白酶无法水解含酪氨酸的肽。胃泌素（人胃蛋白酶C）对这些底物的活性约为猪胃蛋白酶C的三分之一。5. 对于猪胃蛋白酶A和人胃蛋白酶，由单个丙氨酸残基使链延长而导致的水解速率增加最为显著。
相似文献
1
The specificity of some pig and human pepsins towards synthetic peptide substrates.某些猪胃蛋白酶和人胃蛋白酶对合成肽底物的特异性。
Biochem J. 1979 Apr 1;179(1):247-9. doi: 10.1042/bj1790247.
2
The catalytic activity of pig pepsin C towards small synthetic substrates.猪胃蛋白酶C对小型合成底物的催化活性。
Biochem J. 1979 Apr 1;179(1):239-46. doi: 10.1042/bj1790239.
3
The kinetics of hydrolysis of some synthetic substrates containing neutral hydrophilic groups by pig pepsin and chicken liver cathepsin D.猪胃蛋白酶和鸡肝组织蛋白酶D对一些含有中性亲水基团的合成底物的水解动力学
Biochem J. 1983 Apr 1;211(1):237-42. doi: 10.1042/bj2110237.
4
Acyl and amino intermediates in reactions catalysed by pig pepsin. Analysis of transpeptidation products.猪胃蛋白酶催化反应中的酰基和氨基中间体。转肽产物分析。
Biochem J. 1976 Mar 1;153(3):691-9. doi: 10.1042/bj1530691.
5
Roles of Tyr13 and Phe219 in the unique substrate specificity of pepsin B.酪氨酸13和苯丙氨酸219在胃蛋白酶B独特底物特异性中的作用。
Biochemistry. 2006 Dec 5;45(48):14415-26. doi: 10.1021/bi061467a.
6
Comparison of pig and chicken pepsins for protein hydrolysis.猪胃蛋白酶和鸡胃蛋白酶对蛋白质水解作用的比较。
Reprod Nutr Dev. 1999 Jul-Aug;39(4):443-54. doi: 10.1051/rnd:19990404.
7
Oxidation of methionine residues of porcine and bovine pepsins.猪胃蛋白酶和牛胃蛋白酶中甲硫氨酸残基的氧化
Biochemistry. 1975 Feb 11;14(3):631-5. doi: 10.1021/bi00674a026.
8
Peptide inhibitor modified magnetic particles for pepsin separation.用于胃蛋白酶分离的肽抑制剂修饰磁性颗粒
J Sep Sci. 2009 Jun;32(12):2017-21. doi: 10.1002/jssc.200800750.
9
Effects of secondary binding by activator and inhibitor peptides on covalent intermediates of pig pepsin.激活剂和抑制剂肽的二级结合对猪胃蛋白酶共价中间体的影响。
Biochem J. 1976 Mar 1;153(3):701-12. doi: 10.1042/bj1530701.
10
Catalytic properties and chemical composition of pepsins from Atlantic cod (Gadus morhua).大西洋鳕鱼（ Gadus morhua）胃蛋白酶的催化特性和化学成分
Comp Biochem Physiol B. 1990;96(2):323-30. doi: 10.1016/0305-0491(90)90382-4.
引用本文的文献
1
Pepsin Promotes Activation of Epidermal Growth Factor Receptor and Downstream Oncogenic Pathways, at Slightly Acidic and Neutral pH, in Exposed Hypopharyngeal Cells.胃蛋白酶在略微酸性和中性 pH 值下促进表皮生长因子受体及其下游致癌途径在暴露的下咽细胞中的激活。
Int J Mol Sci. 2021 Apr 20;22(8):4275. doi: 10.3390/ijms22084275.
2
Milk Processing Affects Structure, Bioavailability and Immunogenicity of β-lactoglobulin.牛奶加工会影响β-乳球蛋白的结构、生物利用度和免疫原性。
Foods. 2020 Jul 3;9(7):874. doi: 10.3390/foods9070874.
3
Characterization of meprin, a membrane-bound metalloendopeptidase from mouse kidney.小鼠肾脏膜结合金属内肽酶meprin的特性研究。
Biochem J. 1987 Jan 1;241(1):229-35. doi: 10.1042/bj2410229.
4
The catalytic activity of pig pepsin C towards small synthetic substrates.猪胃蛋白酶C对小型合成底物的催化活性。
Biochem J. 1979 Apr 1;179(1):239-46. doi: 10.1042/bj1790239.
本文引用的文献
1
SPECIFICITY OF PEPSIN AND ITS DEPENDENCE ON A POSSIBLE 'HYDROPHOBICBINDING SITE'.胃蛋白酶的特异性及其对可能的“疏水结合位点”的依赖性。
Nature. 1963 Sep 14;199:1094-5. doi: 10.1038/1991094a0.
2
Isolation and crystallization of gastricsin from human gastric juice.从人胃液中分离和结晶胃蛋白酶。
J Biol Chem. 1959 May;234(5):1174-8.
3
Pepsinogen C and pepsin C. Further purification and amino acid composition.胃蛋白酶原C和胃蛋白酶C。进一步纯化及氨基酸组成。
Biochem J. 1966 Oct;101(1):176-83. doi: 10.1042/bj1010176.
4
Hydrolysis of proteins.蛋白质的水解
Adv Protein Chem. 1965;20:37-107. doi: 10.1016/s0065-3233(08)60388-5.
5
The specificity and mechanism of pepsin action.胃蛋白酶作用的特异性及机制。
Adv Enzymol Relat Areas Mol Biol. 1970;33:401-43. doi: 10.1002/9780470122785.ch9.
6
Pepsin D. A minor component of commercial pepsin preparations.胃蛋白酶D。商业胃蛋白酶制剂的一种次要成分。
Biochem J. 1967 Sep;104(3):742-8. doi: 10.1042/bj1040742.
7
The pepsins of human gastric juice. Separation by electrophoresis and chromatography; the influence of gastric disease and ambient pH.人胃液中的胃蛋白酶。通过电泳和色谱法进行分离；胃部疾病和环境pH值的影响。
Scand J Gastroenterol. 1974 Nov;9(8):711-7.
8
The mechanism of the catalytic action of pepsin and related acid proteinases.胃蛋白酶及相关酸性蛋白酶的催化作用机制。
Adv Enzymol Relat Areas Mol Biol. 1976;44:1-36. doi: 10.1002/9780470122891.ch1.
9
The catalytic activity of pig pepsin C towards small synthetic substrates.猪胃蛋白酶C对小型合成底物的催化活性。
Biochem J. 1979 Apr 1;179(1):239-46. doi: 10.1042/bj1790239.
Zotero 插件