Chen Xinyi, Guan Jichen, Zhang Chen, Li Sizheng, Zang Yingda, Chen Jiaqi, Jin Hongtao, Lai Fangfang, Zhang Dongming
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Beijing Union⎼Genius Pharmaceutical Technology Development Co., Ltd., Beijing, 100176, China.
Phytochemistry. 2024 Apr;220:113995. doi: 10.1016/j.phytochem.2024.113995. Epub 2024 Feb 1.
To find structurally previously undescribed compounds with pharmacological effects from Prismatomeris tetrandra (Roxb.) K. Schum (Rubiaceae), thirteen undescribed tetrahydroanthraquinones (1⎼13) named prisconnatanones J⎼V and seven known anthraquinones (14⎼20) were isolated and characterized. The structures of these compounds were elucidated by detailed spectroscopic analyses, and their absolute configurations were established by modified Mosher's method and ECD calculations. The antitumor cell proliferative activities of prisconnatanones J⎼V were determined. Among them, prisconnatanones J possessed high antitumor cell proliferation in HGC27 cells (IC, 0.792 μM) by blocking HGC27 cells in the S phase and significantly inducing apoptosis in HGC27 cells. Prisconnatanone J has no cytotoxicity to normal gastric cells line (GES-1) at 10 μM and showed a considerable selectivity for HGC27 cells. Prisconnatanone J can potentially inhibit tumor cell proliferation and should be further investigated.
为了从茜草科植物棱枝茜草（Prismatomeris tetrandra (Roxb.) K. Schum）中寻找具有药理作用的结构新颖的化合物，我们分离并鉴定了13个未被报道过的四氢蒽醌类化合物（1⎼13），命名为棱枝茜草萘并色原酮J⎼V，以及7个已知的蒽醌类化合物（14⎼20）。通过详细的光谱分析阐明了这些化合物的结构，并采用改进的Mosher方法和ECD计算确定了它们的绝对构型。测定了棱枝茜草萘并色原酮J⎼V的抗肿瘤细胞增殖活性。其中，棱枝茜草萘并色原酮J通过将HGC27细胞阻滞在S期并显著诱导其凋亡，对HGC27细胞具有较高的抗肿瘤细胞增殖活性（IC，0.792 μM）。棱枝茜草萘并色原酮J在10 μM时对正常胃细胞系（GES-1）无细胞毒性，且对HGC27细胞表现出相当的选择性。棱枝茜草萘并色原酮J可能具有抑制肿瘤细胞增殖的作用，值得进一步研究。
