Researcher, UNIP Postgraduation Dental School, Paulista University (UNIP), São Paulo, Brazil.
Researcher, UNIP Postgraduation Dental School, Paulista University (UNIP), São Paulo, Brazil.
J Prosthet Dent. 2024 Oct;132(4):844.e1-844.e6. doi: 10.1016/j.prosdent.2024.01.005. Epub 2024 Feb 2.
Disinfection is an important factor in preserving facial prostheses and maintaining tissue health. However, whether disinfection with ultraviolet C is an effective disinfection method is unclear.
The purpose of this in vitro study was to evaluate the effectiveness of irradiation with different exposure durations of an ultraviolet-C light-emitting diode in the disinfection of the silicone (A-588-1; Factor II) used in facial prostheses.
A total of 216 specimens were prepared, contaminated by multispecies biofilm, and divided into 9 groups (n=24) for different treatments: chlorhexidine 0.12% (G CHG), ultraviolet-C light-emitting diode for 5 minutes (G UVC5), ultraviolet-C light-emitting diode for 10 minutes (G UVC10), ultraviolet-C light-emitting diode for 20 minutes (G UVC20), their respective untreated controls (Gcontrol CHG, Gcontrol UVC5, Gcontrol UVC10, Gcontrol UVC20), and dimethyl sulfoxide (G DMSO) as the negative control. Cell viability was measured by using the methyl tetrazolium salt (MTT) method. Two statistical analyses were performed. First, a 2×3 ANOVA was carried out to compare the control groups (Gcontrol UVC5, Gcontrol UVC10, and Gcontrol UVC20) and the experimental groups of UV-C LED light with different exposure durations (G UVC5, G UVC10, and G UVC20). The second analysis was performed using generalized linear models to compare the optical density of the groups (G UVC5, G UVC10, G UVC20, G CHG, and G DMSO).
Cell viability results demonstrated a microbial reduction after exposure to the ultraviolet-C light-emitting diode for 20 minutes (G UVC20) compared with untreated controls (P<.05). The 5- and 10-minute exposures were statistically similar to their respective control groups (P>.05). The 20 minutes exposure had the lowest average optical density value, being statistically different from the 5-minute exposure (P<.05). A 20-minute exposure to the ultraviolet-C light-emitting diode (G UVC20) was similarly effective when compared with the standard disinfection treatment (G CHG) and dimethyl sulfoxide (G DMSO) (P>.05).
Irradiation with an ultraviolet-C light-emitting diode for 20 minutes decreased the in vitro microbial cell viability on the medical silicone used in facial prostheses.
消毒是保存面部假体和维持组织健康的重要因素。然而，紫外线 C 消毒是否是一种有效的消毒方法尚不清楚。
本体外研究的目的是评估不同紫外线-C 发光二极管照射时间对用于面部假体的硅橡胶（A-588-1；Factor II）消毒的效果。
共制备了 216 个样本，被多菌种生物膜污染，分为 9 组（n=24）进行不同处理：洗必泰 0.12%（G CHG）、紫外线-C 发光二极管照射 5 分钟（G UVC5）、紫外线-C 发光二极管照射 10 分钟（G UVC10）、紫外线-C 发光二极管照射 20 分钟（G UVC20）、各自未处理的对照组（Gcontrol CHG、Gcontrol UVC5、Gcontrol UVC10、Gcontrol UVC20）和二甲基亚砜（G DMSO）作为阴性对照。通过使用甲基噻唑基四唑（MTT）法测量细胞活力。进行了两项统计分析。首先，进行了 2×3 ANOVA 分析，比较了对照组（Gcontrol UVC5、Gcontrol UVC10 和 Gcontrol UVC20）和不同紫外线-C LED 照射时间的实验组（G UVC5、G UVC10 和 G UVC20）。第二次分析使用广义线性模型比较了各组（G UVC5、G UVC10、G UVC20、G CHG 和 G DMSO）的光密度。
细胞活力结果表明，与未处理的对照组相比，暴露于紫外线-C 发光二极管 20 分钟（G UVC20）后微生物减少（P<.05）。5 分钟和 10 分钟的暴露与各自的对照组相比在统计学上相似（P>.05）。20 分钟的暴露具有最低的平均光密度值，与 5 分钟的暴露在统计学上有差异（P<.05）。与标准消毒处理（G CHG）和二甲基亚砜（G DMSO）相比，紫外线-C 发光二极管照射 20 分钟（G UVC20）同样有效（P>.05）。
照射 20 分钟紫外线-C 发光二极管降低了用于面部假体的医用硅橡胶上的体外微生物细胞活力。
