Department of Urology, Hunan Cancer Hospital, the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
NHC Key Laboratory of Human Stem Cell and Reproductive Engineering, Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, Hunan, China.
Sci Data. 2024 Feb 2;11(1):163. doi: 10.1038/s41597-024-02938-5.
Chemotherapeutic drugs will affect the process of spermatogenesis. However, most current studies on the effects of chemotherapeutic drugs on spermatogenesis are based on mouse models, with a shortage of human body evidence. In addition, the mechanism of chemotherapeutic drugs causing spermatogenesis disorder is not clear. Therefore, we have collected the testicular tissues of an inguinal-lipoma patient whose testes were resected after chemotherapy and a patient who had normal spermatogenesis disorder and underwent single-nucleus RNA sequencing (snRNA-Seq). After quality control, we obtained a total of 27,957 high-quality cells, including 18,612 normal cells and 9,345 drug-treated cells, which were all used in analyzing the mechanism of chemotherapeutic drugs causing spermatogenesis disorder. This study has provided data resources and references for exploring the mechanism of chemotherapeutic drugs causing spermatogenesis disorder with the insight of protecting the spermatogenic abilities of male tumor patients receiving chemotherapy.
化疗药物会影响精子发生过程。然而，目前大多数关于化疗药物对精子发生影响的研究都是基于小鼠模型，缺乏人体证据。此外，化疗药物导致精子发生障碍的机制尚不清楚。因此，我们收集了一名腹股沟脂肪瘤患者的睾丸组织，该患者在化疗后接受了睾丸切除术，以及一名精子发生正常但存在障碍的患者的睾丸组织，并进行了单细胞 RNA 测序 (snRNA-Seq)。经过质量控制，我们共获得了 27957 个高质量细胞，包括 18612 个正常细胞和 9345 个药物处理细胞，这些细胞均用于分析化疗药物导致精子发生障碍的机制。这项研究为探索化疗药物导致精子发生障碍的机制提供了数据资源和参考，为保护接受化疗的男性肿瘤患者的生精能力提供了新的思路。
