Lin Jinhui, Li Siyaqi, Ying Yunfei, Zheng Weilin, Wu Jingcheng, Wang Peiyuan, Liu Xiaolong
Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, P. R. China.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
ACS Omega. 2024 Jan 16;9(4):4386-4394. doi: 10.1021/acsomega.3c06683. eCollection 2024 Jan 30.
Triggering the healing process of drug-resistant bacteria-infected wounds has attracted great attention due to global morbidity that may induce gangrene, amputation, and even death. Here, a chitin derivative, carboxymethyl chitosan (CMC), tannic acid (TA), and Cu were used for hydrogel engineering. Using sodium bicarbonate as the neutralizer and reductant, hydrogen bonds between CMC and TA and in situ Cu(OH) generation via ion coordination force between Cu and TA facilitated the synthesis of CMC/TA/Cu hydrogel. Cu and TA release, cytotoxicity, in vitro cell migration, angiogenesis, and antidrug-resistant bacteria were measured. Besides, wound closure was evaluated in vivo using the methicillin-resistant (MRSA)-infected excisional dermal wound mouse model. Negligible toxicity was observed both in vitro and in vivo. Dermal cell migration and angiogenesis were significantly enhanced. In vivo, the CMC/TA/Cu hydrogel induced effective re-epithelialization, collagen deposition, inflammatory alleviation, and MRSA inhibition during wound repair in mice. All these results confirmed that the CMC/TA/Cu hydrogel is a promising novel dressing for chronic wound healing in clinic.
由于耐药菌感染伤口引发的全球发病率可能导致坏疽、截肢甚至死亡，触发其愈合过程已引起了广泛关注。在此，壳聚糖衍生物羧甲基壳聚糖（CMC）、单宁酸（TA）和铜被用于水凝胶工程。以碳酸氢钠作为中和剂和还原剂，CMC与TA之间的氢键以及通过铜与TA之间的离子配位作用原位生成的氢氧化铜促进了CMC/TA/Cu水凝胶的合成。对铜和TA的释放、细胞毒性、体外细胞迁移、血管生成及抗耐药菌能力进行了测定。此外，使用耐甲氧西林金黄色葡萄球菌（MRSA）感染的切除性皮肤伤口小鼠模型在体内评估了伤口闭合情况。在体外和体内均观察到可忽略不计的毒性。真皮细胞迁移和血管生成显著增强。在体内，CMC/TA/Cu水凝胶在小鼠伤口修复过程中诱导了有效的上皮再形成、胶原蛋白沉积、炎症减轻以及MRSA抑制。所有这些结果证实，CMC/TA/Cu水凝胶是临床上用于慢性伤口愈合的一种有前景的新型敷料。
