Mapp Biopharmaceutical, San Diego, CA, USA.
Methods Mol Biol. 2024;2762:183-190. doi: 10.1007/978-1-0716-3666-4_11.
Antibodies are versatile biological molecules with widespread applications in research and medicine. This protocol outlines the generation of monoclonal IgG antibodies from Chinese hamster ovary cells. It includes steps for cell maintenance, transient transfection, and antibody purification via protein A affinity chromatography. The methods described are intended for the production of milligram amounts of protein but can be adapted for most small- to mid-scale applications.
抗体是用途广泛的生物分子，在研究和医学领域有广泛的应用。本方案概述了从中国仓鼠卵巢细胞中产生单克隆 IgG 抗体的过程。它包括细胞维持、瞬时转染以及通过蛋白 A 亲和层析进行抗体纯化的步骤。所描述的方法旨在生产毫克量的蛋白质，但可以适应大多数小规模至中等规模的应用。
