Li Heqiang, Gao Jing, Wang Jingyi, Zhao Baobao, Ma Xiaonan, Zhang Bihan, Lv Changrong, Qiao Hailian, Wang Yongsheng, Qing Suzhu
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, 712100, PR China.
Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Shaanxi, 712100, PR China.
Biol Reprod. 2024 Feb 6. doi: 10.1093/biolre/ioae024.
Accumulating evidence indicates that paternally-derived miRNAs play a crucial role in the development of early embryos and are regarded as the key factor in the successful development of somatic cell cloned embryos. In our previous study, bta-miR-301a was found to be highly expressed in bovine sperm, and was delivered into oocytes during fertilization. In this study, bioinformatics, dual luciferase reporter assays, rescue experiments and gain- and loss-of-function experiments indicated that ACVR1 is the target gene of bta-miR-301a in early bovine embryos. By microinjecting bta-miR-301a mimic into embryos of parthenogenetic or somatic cell nuclear transfer, we observed that bta-miR-301a prolonged the first cleavage time of the embryos and increased the blastocyst formation rate. Thus, this study provides preliminary evidence that bta-miR-301a influences remodeling of the microfilament skeleton, prolongs the first cleavage time, and improves the developmental competence of embryos by negatively regulating ACVR1 translation.
越来越多的证据表明，父源miRNA在早期胚胎发育中起关键作用，被视为体细胞克隆胚胎成功发育的关键因素。在我们之前的研究中，发现bta-miR-301a在牛精子中高表达，并在受精过程中传递到卵母细胞中。在本研究中，生物信息学、双荧光素酶报告基因检测、拯救实验以及功能获得和缺失实验表明，ACVR1是早期牛胚胎中bta-miR-301a的靶基因。通过将bta-miR-301a模拟物显微注射到孤雌或体细胞核移植胚胎中，我们观察到bta-miR-301a延长了胚胎的首次卵裂时间，并提高了囊胚形成率。因此，本研究提供了初步证据，表明bta-miR-301a通过负调控ACVR1翻译来影响微丝骨架的重塑，延长首次卵裂时间，并提高胚胎的发育能力。
