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蛋白质酪氨酸磷酸酶PPH - 7是秀丽隐杆线虫在高温下生育力和胚胎发育所必需的。

The protein tyrosine phosphatase PPH-7 is required for fertility and embryonic development in C. elegans at elevated temperatures.

作者信息

Franziscus Curdin A, Ritz Danilo, Kappel N Constantin, Solinger Jachen A, Schmidt Alexander, Spang Anne

机构信息

Biozentrum, University of Basel, Switzerland.

Leica Microsystems CMS GmbH, Wetzlar, Germany.

出版信息

FEBS Open Bio. 2024 Mar;14(3):390-409. doi: 10.1002/2211-5463.13771. Epub 2024 Feb 6.

DOI:10.1002/2211-5463.13771
PMID:38320757
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10909979/
Abstract

Post-translational modifications are key in the regulation of activity, structure, localization, and stability of most proteins in eukaryotes. Phosphorylation is potentially the most studied post-translational modification, also due to its reversibility and thereby the regulatory role this modification often plays. While most research attention was focused on kinases in the past, phosphatases remain understudied, most probably because the addition and presence of the modification is more easily studied than its removal and absence. Here, we report the identification of an uncharacterized protein tyrosine phosphatase PPH-7 in C. elegans, a member of the evolutionary conserved PTPN family of phosphatases. Lack of PPH-7 function led to reduction of fertility and embryonic lethality at elevated temperatures. Proteomics revealed changes in the regulation of targets of the von Hippel-Lindau (VHL) E3 ligase, suggesting a potential role for PPH-7 in the regulation of VHL.

摘要

翻译后修饰是真核生物中大多数蛋白质的活性、结构、定位和稳定性调节的关键。磷酸化可能是研究最多的翻译后修饰，这也归因于其可逆性以及这种修饰经常发挥的调节作用。虽然过去大多数研究注意力集中在激酶上，但磷酸酶仍未得到充分研究，很可能是因为修饰的添加和存在比其去除和不存在更容易研究。在这里，我们报告了在秀丽隐杆线虫中鉴定出一种未表征的蛋白酪氨酸磷酸酶PPH-7，它是进化保守的磷酸酶PTPN家族的成员。PPH-7功能的缺失导致在高温下生育力降低和胚胎致死。蛋白质组学揭示了冯·希佩尔-林道（VHL）E3连接酶靶标的调节变化，表明PPH-7在VHL调节中可能发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0dba/10909979/862040831664/FEB4-14-390-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0dba/10909979/ae6f1e6afd97/FEB4-14-390-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0dba/10909979/3c578d4e3af4/FEB4-14-390-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0dba/10909979/c1c394688c60/FEB4-14-390-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0dba/10909979/20a1bd053c05/FEB4-14-390-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0dba/10909979/862040831664/FEB4-14-390-g003.jpg

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