Department of Experimental and Clinical Medicine, University of Florence, Viale Morgagni 50, 50134 Florence, Italy.
Department of Experimental and Clinical Biomedical Sciences "Mario Serio", Italy.
Biochim Biophys Acta Rev Cancer. 2020 Dec;1874(2):188442. doi: 10.1016/j.bbcan.2020.188442. Epub 2020 Oct 2.
In mammalian cells, tyrosine phosphorylation is one of the main mechanisms responsible for regulating signal transduction pathways and key cellular functions. Moreover, recent studies demonstrated that tyrosine phosphorylation influences the activity of some metabolic enzymes, even if it remains to be clarified whether tyrosine phosphorylation can be considered a general mechanism involving most of the metabolic enzymes or only a subset of these. To elucidate this aspect, we conducted a two-step analysis. First, we analyzed literature to identify all the metabolic enzymes whose activity is affected by tyrosine phosphorylation. Second, we crossed these data with those obtained from the PhosphoSitePlus database analysis. Collected information was used to depict an exhaustive map showing the real spread of tyrosine phosphorylation among metabolic enzymes. In summary, data reported in this review highlight that tyrosine phosphorylation is not a sporadic event but a widespread post-translational modification, which is essential to promote the metabolic reprogramming of cancer cells.
在哺乳动物细胞中，酪氨酸磷酸化是负责调节信号转导途径和关键细胞功能的主要机制之一。此外，最近的研究表明，酪氨酸磷酸化影响一些代谢酶的活性，尽管尚不清楚酪氨酸磷酸化是否可以被认为是一种涉及大多数代谢酶的通用机制，还是这些酶的一个子集。为了阐明这一方面，我们进行了两步分析。首先，我们分析了文献，以确定所有其活性受酪氨酸磷酸化影响的代谢酶。其次，我们将这些数据与从 PhosphoSitePlus 数据库分析中获得的数据进行交叉。收集到的信息被用来描绘一张详尽的地图，显示酪氨酸磷酸化在代谢酶中的实际分布。总之，本综述中报告的数据强调了酪氨酸磷酸化不是一个偶然事件，而是一种广泛存在的翻译后修饰，对促进癌细胞的代谢重编程至关重要。
