Kakinuma Kaoru, Kakinuma Toshiyuki
Department of Obstetrics and Gynecology, International University of Health and Welfare Hospital, Tochigi 329-2763, Japan.
Graduate School of Medicine, International University of Health and Welfare, Tokyo 107-8402, Japan.
World J Clin Cases. 2024 Jan 26;12(3):479-487. doi: 10.12998/wjcc.v12.i3.479.
Premature ovarian insufficiency (POI) is a condition that causes secondary amenorrhea owing to ovarian hypofunction at an early stage. Early follicular depletion results in intractable infertility, thereby considerably reducing the quality of life of females. Given the continuum in weakened ovarian function, progressing from incipient ovarian failure (IOF) to transitional ovarian failure and further to POI, it is necessary to develop biomarkers for predicting POI. The oxidative stress states in IOF and POI were comprehensively evaluated oxidative stress [diacron-reactive oxygen metabolites (d-ROMs)] test and antioxidant capacity [biological antioxidant potential (BAP)].
To explore the possibilities of oxidative stress and antioxidant capacity as biomarkers for the early detection of POI.
Females presenting with secondary amenorrhea over 4 mo and a follicle stimulating hormone level of > 40 mIU/mL were categorized into the POI group. Females presenting with a normal menstrual cycle and a follicle stimulating hormone level of > 10.2 mIU/mL were categorized into the IOF group. Healthy females without ovarian hypofunction were categorized into the control group. Among females aged < 40 years who visited our hospital from January 2021 to June 2022, we recruited 11 patients into both POI and IOF groups. For the potential antioxidant capacity, the relative oxidative stress index (BAP/d-ROMs × 100) was calculated, and the oxidative stress defense system was comprehensively evaluated.
d-ROMs were significantly higher in the POI and IOF groups than in the control group, (478.2 ± 58.7 U.CARR, 434.5 ± 60.6 U.CARR, and 341.1 ± 35.1 U.CARR, respectively) (U.CARR is equivalent to 0.08 mg/dL of hydrogen peroxide). However, no significant difference was found between the POI and IOF groups. Regarding BAP, no significant difference was found between the control, IOF, and POI groups (2078.5 ± 157.4 μmol/L, 2116.2 ± 240.2 μmol/L, and 2029.0 ± 186.4 μmol/L, respectively). The oxidative stress index was significantly higher in the POI and IOF groups than in the control group (23.7 ± 3.3, 20.7 ± 3.6, and 16.5 ± 2.1, respectively). However, no significant difference was found between the POI and IOF groups.
High levels of oxidative stress suggest that evaluating the oxidative stress state may be a useful indicator for the early detection of POI.
卵巢早衰（POI）是一种由于早期卵巢功能减退导致继发性闭经的病症。早期卵泡耗竭会导致难以治愈的不孕症，从而显著降低女性的生活质量。鉴于卵巢功能从初期卵巢功能衰竭（IOF）逐渐发展为过渡性卵巢功能衰竭进而发展为POI的连续性，有必要开发预测POI的生物标志物。通过氧化应激[戴克隆活性氧代谢物（d-ROMs）]试验和抗氧化能力[生物抗氧化潜能（BAP）]对IOF和POI中的氧化应激状态进行了综合评估。
探讨氧化应激和抗氧化能力作为早期检测POI生物标志物的可能性。
将出现继发性闭经超过4个月且促卵泡生成素水平>40 mIU/mL的女性归入POI组。将月经周期正常且促卵泡生成素水平>10.2 mIU/mL的女性归入IOF组。无卵巢功能减退的健康女性归入对照组。在2021年1月至2022年6月到我院就诊的年龄<40岁的女性中，我们在POI组和IOF组各招募了11例患者。对于潜在的抗氧化能力，计算相对氧化应激指数（BAP/d-ROMs×100），并对氧化应激防御系统进行综合评估。
POI组和IOF组的d-ROMs显著高于对照组，分别为（478.2±58.7 U.CARR、434.5±60.6 U.CARR和341.1±35.1 U.CARR）（U.CARR相当于0.08 mg/dL的过氧化氢）。然而，POI组和IOF组之间未发现显著差异。关于BAP，对照组、IOF组和POI组之间未发现显著差异，分别为（2078.5±157.4 μmol/L、2116.2±240.2 μmol/L和2029.0±186.4 μmol/L）。POI组和IOF组的氧化应激指数显著高于对照组，分别为（23.7±3.3、20.7±3.6和16.5±2.1）。然而，POI组和IOF组之间未发现显著差异。
高水平的氧化应激表明，评估氧化应激状态可能是早期检测POI的有用指标。
Zotero 插件